SURFACE PROPERTIES OF NEISSERIA-GONORRHOEAE - ISOLATION OF MAJOR COMPONENTS OF OUTER MEMBRANE
SURFACE PROPERTIES OF NEISSERIA-GONORRHOEAE - ISOLATION OF MAJOR COMPONENTS OF OUTER MEMBRANE
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DOI：
10.1099/00221287-99-2-333
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
HECKELS, JE
中科院分区：
文献类型：
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作者：
HECKELS, JE
从几株N.通过用乙酸锂水溶液提取全细胞来检测淋病。制剂含有有限数量的组分，包括脂多糖和2种主要蛋白质。一种蛋白存在于所有菌株检查;第二，这表明异常行为的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，是从一些缺席。荧光标记完整的N.淋病奈瑟菌的表面蛋白质分析表明，这2种蛋白质和皮利是主要的表面蛋白质。通过选择性提取，然后进行凝胶过滤，以均质形式分离3种主要外膜组分。
Outer membranes were isolated from several strains of N. gonorrhoeae by extraction of whole cells with aqueous lithium acetate. The preparations contained a limited number of components including lipopolysaccharide and 2 major proteins. One protein was present in all strains examined; the 2nd, which showed anomalous behavior on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was absent from some. Fluorescent labeling of intact N. gonorrhoeae showed that the 2 proteins and pili were the major surface proteins. Each of the 3 major outer membrane components was isolated in a homogeneous form by selective extraction followed by gel filtration.