Glucose transporter 5 (GLUT5)-like immunoreactivity is localized in subsets of neurons and glia in the rat brain
Glucose transporter 5 (GLUT5)-like immunoreactivity is localized in subsets of neurons and glia in the rat brain
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DOI：
10.1016/j.jchemneu.2016.03.004
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发表时间：
2016-07-01
影响因子：
2.8
通讯作者：
Yamamoto, Toshiharu
中科院分区：
文献类型：
--
作者：
Kojo, Akiko;Yamada, Kentaro;Yamamoto, Toshiharu
本研究旨在通过免疫组织化学和Western blotting检测优先转运果糖的葡萄糖转运蛋白5(GLUT5)在大鼠脑内的分布。免疫反应阳性的小斑点(小于0.7微米)稀疏分布于脑内，其中一些似乎与小胶质细胞突起有关，抗钙结合适配器分子1(IBA-1)单抗检测到的小胶质细胞突起。此外，这些免疫反应点中的一些似乎与抗胶质纤维酸性蛋白(GFAP)单抗标记的Tanycell突起有关。室管膜细胞也呈GLUT5免疫阳性。此外，观察到了几个值得注意的GLUT免疫反应特征。GLUT5免疫反应阳性神经元可见于延髓内侧核和下丘脑外侧核。小脑浦肯野细胞GLUT5免疫阳性。在视束及其终场，即上丘、顶盖前、视束核和视束内侧终核，可观察到免疫反应阳性斑点密集聚集，其中一些为神经元成分(经免疫电子显微镜证实)。除视觉系统的相关区域外，前庭核和耳蜗核也含有密集的GLUT5免疫反应点。小脑蛋白质印迹分析表明，所用抗体识别空肠和肾提取液中也含有的33.5和37.0 kDa条带。因此，这些结果表明，谷氨酸可能在胶质细胞和神经元的亚群中运输果糖，作为这些细胞的能量来源。(C)2016爱思唯尔B.V.保留所有权利。
This study aimed at examining the distribution of glucose transporter 5 (GLUT5), which preferentially transports fructose, in the rat brain by immunohistochemistry and Western blotting. Small immunoreactive puncta (less than 0.7 mu m) were sparsely distributed all over the brain, some of which appeared to be associated with microglial processes detected by an anti-ionized calcium-binding adapter molecule 1 (Iba-1) monoclonal antibody. In addition, some of these immunoreactive puncta seemed to be associated with tanycyte processes that were labeled with anti-glial fibrillary acidic protein (GFAP) monoclonal antibody. Ependymal cells were also found to be immunopositive for GLUT5. Furthermore, several noticeable GLUTS immunoreactive profiles were observed. GLUT5 immunoreactive neurons, confirmed by double staining with neuronal nuclei (NeuN), were seen in the entopeduncular nucleus and lateral hypothalamus. Cerebellar Purkinje cells were immunopositve for GLUT5. Dense accumulation of immunoreactive puncta, some of which were neuronal elements (confirmed by immunoelectron microscopy), were observed in the optic tract and their terminal fields, namely, superior colliculus, pretectum, nucleus of the optic tract, and medial terminal nucleus of the optic tract. In addition to the associated areas of the visual system, the vestibular and cochlear nuclei also contained dense GLUT5 immunoreactive puncta. Western blot analysis of the cerebellum indicated that the antibody used recognized the 33.5 and 37.0 kDa bands that were also contained in jejunum and kidney extracts. Thus, these results suggest that GLUTS may transport fructose in subsets of the glia and neurons for an energy source of these cells. (C) 2016 Elsevier B.V. All rights reserved.