Effect of Ca2+-free and Mg2+-free BSS plus solution on the retinal pigment epithelium and retina in rabbits
Effect of Ca2+-free and Mg2+-free BSS plus solution on the retinal pigment epithelium and retina in rabbits
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DOI：
10.1016/s0002-9394(00)00899-0
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发表时间：
2001-04-01
影响因子：
4.2
通讯作者：
Tano, Y
中科院分区：
文献类型：
--
作者：
Fang, XY;Hayashi, A;Tano, Y
目的：测定无Ca2+和无Mg2+ BSS Plus（爱尔康实验室，Fort Worth, Texas）溶液玻璃体内冲洗是否改变兔视网膜色素上皮与视网膜之间的粘附性。方法：对34只荷兰色素兔34只眼进行晶状体切除术和玻璃体切除术。随后，用不含Ca2+和Mg2+的BSS Plus溶液冲洗24只眼的玻璃体腔10或20分钟。另外10只眼用BSS +溶液冲洗20分钟作为对照。为了确定视网膜色素上皮与视网膜之间的粘附性，在34只眼睛中有12只产生视网膜脱离。扫描电镜观察视网膜色素上皮顶端表面和光感受器外节，视网膜电图观察视网膜生理，光镜观察视网膜形态学。结果:视网膜脱离发生后，用不含Ca2+和Mg2+的BSS Plus溶液冲洗20分钟后，观察视网膜色素上皮顶端表面的锥体鞘数(33 +/- 15，平均+/- SD)显著低于BSS +溶液冲洗20分钟后眼视网膜色素上皮顶表面锥体鞘数（120 +/- 50）或无Ca2+和Mg2+ BSS +溶液冲洗10分钟后眼视网膜色素上皮顶表面锥体鞘数（115 +/- 49;P = 0.02）。无Ca2+和Mg2+ BSS Plus溶液冲洗20分钟后，与BSS Plus溶液冲洗20分钟后相比，术后第1天眼内b波振幅降低（P = 0.03）。术后第3天，两组b波差异无统计学意义（P < 0.05）。光镜检查显示两种溶液使用后没有形态学异常。结论：用无Ca2+和Mg2+的BSS +溶液在玻璃体内冲洗20分钟，可改变视网膜色素上皮微绒毛与视网膜外段的粘连，减轻视网膜脱离的创伤性。这些结果表明，无Ca2+和无Mg2+的BSS +溶液可能对中央凹移位手术中故意视网膜脱离的产生具有临床价值。[J]中华眼科杂志，2001;31(1):481-488。(C) 2001年Elsevier Science Inc.版权所有)。
PURPOSE: To determine whether intravitreal irrigation with Ca2+-free and Mg2+-free BSS Plus (Alcon Laboratory, Fort Worth, Texas) solution alters the adhesiveness between the retinal pigment epithelium and the retina of rabbits.METHODS: Thirty-four eyes of 34 Dutch pigmented rabbits underwent lensectomy and vitrectomy. Subsequently, the vitreous cavity of 24 eyes was irrigated with Ca2+-free and Mg2+-free BSS Plus solution for 10 or 20 minutes. The other 10 eyes were irrigated with BSS Plus solution for 20 minutes as controls. To determine the adhesiveness between the retinal pigment epithelium and retina, a retinal detachment was produced in 12 of the 34 eyes. The apical surface of the retinal pigment epithelium and the photoreceptor outer segments were examined by scanning electron microscopy, Retinal physiology was assessed by electroretinography and retinal morphology by light microscopy.RESULTS: After retinal detachment was produced, the number of cone sheaths on the apical surface of the retinal pigment epithelium after irrigation with Ca2+-free and Mg2+-free BSS Plus solution for 20 minutes (33 +/- 15, mean +/- SD) was significantly less than the number of cone sheaths on the apical surface of the retinal pigment epithelium of eyes after irrigation with BSS Plus solution for 20 minutes (120 +/- 50) or the number of cone sheaths on the apical surface of the retinal pigment epithelium of eyes after 10 minutes of irrigation with Ca2+-free and Mg2+-free BSS Plus solution (115 +/- 49; P = .02). The b-wave amplitudes in the eyes irrigated with Ca2+-free and Mg2+-free BSS Plus solution for 20 minutes were depressed compared with the b-waves in eyes irrigated with BSS Plus solution for 20 minutes on the first postoperative day (P = .03). After the third postoperative day, there was no significant difference in the b-waves (P > .06). Light microscopy demonstrated no morphologic abnormalities after the use of both solutions.CONCLUSIONS: Intravitreal irrigation with Ca2+-free and Mg2+-free BSS Plus solution for 20 minutes altered the adhesion between the retinal pigment epithelium microvilli and retinal outer segments and made the creation of retinal detachment less traumatic. These results suggest that Ca2+-free and Mg2+-free BSS Plus solution may be of clinical value for the creation of an intentional retinal detachment for foveal translocation surgery. (Am J Ophthalmol 2001;131:481-488. (C) 2001 by Elsevier Science Inc. All rights reserved).