Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources
Assessment of Specimen Pooling to Conserve SARS CoV-2 Testing Resources
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DOI：
10.1093/ajcp/aqaa064
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发表时间：
2020-06-01
影响因子：
3.5
通讯作者：
Iwen, Peter C.
中科院分区：
文献类型：
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作者：
Abdalhamid, Baha;Bilder, Christopher R.;Iwen, Peter C.
目的：目的：建立SARS冠状病毒2型（SARS-CoV-2）混合样本分组检测的最佳参数。从该分析中，使用50 μ L来自一个SARS-CoV-2阳性鼻咽样本的样本与4个阴性患者样本（各50 μ L）混合，总体积为250 μ L，创建25个实验池。随后从每个样本池中提取病毒RNA，并使用CDC SARS-CoV-2 RT-PCR检测试剂盒进行检测。随后将阳性样本池分成单个标本，并通过提取和PCR进行检测。结果：25个样本库均为阳性，其循环阈值（Ct）在0 ~ 5.03Ct范围内。对60份标本的分析确定2份样本池为阳性，随后在60份供试标本中识别出2份个体标本。本测试完成，同时使用22提取/PCR测试，节省38 reactions.Conclusions：当SARS-CoV-2感染的发病率为10%或更低，组测试将导致试剂和人员的时间节省与整体增加至少69%的测试能力。
Objectives: To establish the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2.Methods: The most efficient pool size was determined to be five specimens using a web-based application. From this analysis, 25 experimental pools were created using 50 mu L from one SARS-CoV-2 positive nasopharyngeal specimen mixed with 4 negative patient specimens (50 mu L each) for a total volume of 250 mu L. Viral RNA was subsequently extracted from each pool and tested using the CDC SARS-CoV-2 RT-PCR assay. Positive pools were consequently split into individual specimens and tested by extraction and PCR. This method was also tested on an unselected group of 60 nasopharyngeal specimens grouped into 12 pools.Results: All 25 pools were positive with cycle threshold (Ct) values within 0 and 5.03 Ct of the original individual specimens. The analysis of 60 specimens determined that 2 pools were positive followed by identification of 2 individual specimens among the 60 tested. This testing was accomplished while using 22 extractions/PCR tests, a savings of 38 reactions.Conclusions: When the incidence rate of SARS-CoV-2 infection is 10% or less, group testing will result in the saving of reagents and personnel time with an overall increase in testing capability of at least 69%.