Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse.
Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse.
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DOI：
10.1093/intimm/1.4.321
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发表时间：
1989-01-01
影响因子：
4.4
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Forster, I;Vieira, P;Rajewsky, K
采用流式细胞术同时检测细胞表面标志物和5-溴-2‘-脱氧尿嘧啶核苷(BrdU)掺入的方法，研究了B系亚群在正常成年小鼠体内的分布和增殖行为。在骨髓中，我们可以根据PAN-B细胞标记物B220和膜相关的MU和Delta免疫球蛋白重链的差异表达来定义B细胞的两个亚群。B220迟钝MU+Delta-B细胞来源于快速分裂的细胞，可能代表B220迟钝MU-Pre-B细胞新近产生的B细胞。相比之下，在B220明亮的MU+Delta+B细胞中，只有少数(如果有的话)在8天后被BrdU标记，这表明这些细胞代表着驻留在骨髓中的长寿B细胞。BrdU掺入脾B细胞的分析表明，其中只有20%的细胞在前8天经历了细胞分裂。在8月龄的动物中，几乎没有腹膜中的B细胞在7天内被BrdU标记，其中很大一部分属于Ly1B亚群。
Using a method which allows simultaneous flow cytometric detection of cell surface markers and 5-bromo-2'-deoxyuridine (BrdU) incorporation, the distribution and proliferative behavior of B lineage subpopulations was studied in intact adult mice. In the bone marrow we could define two subsets of B cells on the basis of differential expression of the pan-B cell marker B220 and of membrane-associated mu and delta immunoglobulin heavy chains. B220dull mu+ delta- B cells were found to emerge from rapidly dividing cells and probably represent B cells recently generated from B220dull mu- pre-B cells. In contrast, only few, if any, of the B220bright mu+ delta+ B cells were labeled with BrdU after a period of 8 days, suggesting that these cells represent long-lived B cells residing in the bone marrow. Analysis of BrdU-incorporation into splenic B cells showed that only 20% of these cells had gone through cell division during the preceding 8 days. Almost none of the B cells in the peritoneum, a large fraction of which belongs to the Ly1 B subset, were labeled with BrdU over a period of 7 days in 8-month-old animals.