Sensitivity analysis of a multibranched light guide for real time hyperspectral imaging systems.
Sensitivity analysis of a multibranched light guide for real time hyperspectral imaging systems.
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用于实时高光谱成像系统的多分支光导的灵敏度分析。
DOI：
10.1117/12.2510506
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Leavesley,SilasJ
中科院分区：
文献类型：
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作者：
Browning,CraigM;Mayes,Samuel;Deal,Joshua;Arshad,Arslan;Mayes,SamanthaGunn;Parker,Marina;Rich,ThomasC;Leavesley,SilasJ
高光谱成像（HSI）是一种光谱技术，它在宽波长范围内捕获高对比度的图像，以显示像素特定的组成。HSI的传统用途包括：卫星图像、食品分发质量控制和数字考古重建。我们的实验室专注于开发HSI荧光成像系统的应用，以研究快速细胞信号事件或实时内窥镜筛查的分子特异性检测。之前，我们已经开发了一个原型光谱光源，使用我们改进的成像技术，激发扫描高光谱成像（HIFEX），结合商业结肠镜进行可行性测试。16波长LED阵列组合，使用多分支固体光导，耦合到瞄准镜的光输入。原型以接近视频速率的速度（~ 8 fps）获得频谱扫描。原型可以以非常快的波长开关速度工作，限制在led的开/关速率（~ 10 μs），但由于通过固体光导的光传输损失（~ 98%），成像速度受到限制。在这里，我们继续我们以前的工作，对固体光导进行深入分析，以优化光强吞吐量。评估的参数包括：LED强度输入，几何形状（分支曲率和组合）和使用外包层的光传播。利用蒙特卡罗射线追踪软件（TracePro）进行了模拟。结果表明，通过LED聚焦透镜、弯曲半径和光滑的切向分支合并可以优化分支光导内的传输。未来的工作将从优化的模型框架中测试新的制造光导。
Hyperspectral imaging (HSI) is a spectroscopic technique which captures images at a high contrast over a wide range of wavelengths to show pixel specific composition. Traditional uses of HSI include: satellite imagery, food distribution quality control and digital archaeological reconstruction. Our lab has focused on developing applications of HSI fluorescence imaging systems to study molecule-specific detection for rapid cell signaling events or real-time endoscopic screening. Previously, we have developed a prototype spectral light source, using our modified imaging technique, excitation-scanning hyperspectral imaging (HIFEX), coupled to a commercial colonoscope for feasibility testing. The 16 wavelength LED array was combined, using a multi-branched solid light guide, to couple to the scope’s optical input. The prototype acquired a spectral scan at near video-rate speeds (∼8 fps). The prototype could operate at very rapid wavelength switch speeds, limited to the on/off rates of the LEDs (∼10 μs), but imaging speed was limited due to optical transmission losses (∼98%) through the solid light guide. Here we present a continuation of our previous work in performing an in-depth analysis of the solid light guide to optimize the optical intensity throughput. The parameters evaluated include: LED intensity input, geometry (branch curvature and combination) and light propagation using outer claddings. Simulations were conducted using a Monte Carlo ray tracing software (TracePro). Results show that transmission within the branched light guide may be optimized through LED focusing lenses, bend radii and smooth tangential branch merges. Future work will test a new fabricated light guide from the optimized model framework.