Micropeptide vsp21 translated by Reovirus circular RNA 000048 attenuates viral replication
Micropeptide vsp21 translated by Reovirus circular RNA 000048 attenuates viral replication
复制标题
由呼肠孤病毒环状 RNA 000048 翻译的微肽 vsp21 减弱病毒复制
DOI：
10.1016/j.ijbiomac.2022.04.136
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发表时间：
2022
影响因子：
8.2
通讯作者：
Chengliang Gong
中科院分区：
文献类型：
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作者：
Yunshan Zhang;Min Zhu;Xing Zhang;Kun Dai;Zi Liang;Jun Pan;Ziyao Zhang;Manman Cao;Renyu Xue;Guangli Cao;Xiaolong Hu;Chengliang Gong
迄今为止，已经发现一些DNA病毒和单链RNA病毒产生circRNA。然而，关于双链RNA病毒产生的circRNA的报道非常有限。本研究证明呼肠孤病毒科的一种典型的双链RNA病毒-（vcircRNA）和vcircRNA_000048，其序列对应于BmCPV基因组dsRNA S5区段上的164-1245 nt区域通过PCR、桑格测序、逆转录-滚环扩增和北方印迹法验证（GQ 294468.1）。此外，我们验证了vcircRNA_000048以IRES依赖的方式翻译具有21个氨基酸残基的微肽vsp 21，并且vsp 21减弱病毒复制。这些发现为了解呼肠孤病毒的增殖调控及与宿主的相互作用提供了新的线索。
To date, some DNA viruses and single-stranded RNA viruses have been found to generate circRNAs. However, the reports on circRNAs produced by double-stranded RNA viruses are very limited. In this study,Bombyx moricypovirus (BmCPV), a typical double-stranded RNA virus belonging to theReoviridae,was demonstrated to generate viral circRNAs (vcircRNAs) and a vcircRNA_000048 whose sequence corresponds with the region 164–1245 nt on the BmCPV genomic dsRNA S5 segment (GQ294468.1) was validated by PCR, Sanger sequencing, reverse transcription-rolling circle amplification, and Northern blotting. Furthermore, we verified that vcircRNA_000048 translates a micropeptide vsp21 with 21 amino acid residues in an IRES-dependent manner, and vsp21 attenuates the viral replication. These findings provided a novel clue to understanding the regulation of viral multiplication and interaction of reovirus with the host.