Simulation of Drug Release from PLGA Particles In Vivo.
Simulation of Drug Release from PLGA Particles In Vivo.
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DOI：
10.1155/2013/513950
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发表时间：
2013
影响因子：
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通讯作者：
Fukushima K
中科院分区：
文献类型：
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作者：
Sasaki K;Igarashi M;Hinata M;Komori Y;Fukushima K
组织和/或细胞的特异性靶向对于任何类型的药物递送系统都是必不可少的，因为这决定了药物的功效和副作用。聚乳酸-羟基乙酸共聚物（PLGA）具有良好的生物相容性和生物降解性，长期以来一直被用作药物释放的生物材料。PLGA颗粒的直接可视化即使在组织内也是可行的，并且药物递送系统的细胞特异性通常通过使用标记的颗粒来评估。然而，单独的颗粒标记并不能解决诸如药物的释放和分布等因素。因此，需要建立一个药物在体内释放和分布的模拟系统。本研究以Hoechst 33342为模拟药物，建立了一种模拟药物在PLGA载体中分布的方法。我们的方法使我们能够识别，分离和表征暴露于Hoechst 33342的细胞，并推导出靶细胞和非靶细胞周围的荧光染料浓度。我们相信，本文所述的方法将提供关于任何类型的PLGA药物递送系统中细胞靶向特异性的基本信息。
Specific targeting of tissues and/or cells is essential for any type of drug delivery system because this determines the efficacy and side effects of the drug. Poly lactic-co-glycolic acids (PLGA) have long been used as biomaterials for drug delivery due to their excellent biocompatibility and biodegradability. Direct visualization of PLGA particles is feasible even within tissues, and cell specificity of the drug delivery system is normally assessed by using labeled particles. However, particle labeling alone does not address factors such as the release and distribution of the drug. Thus, it is desirable to set up a simulation system of drug release and distribution in vivo. In the present study, we aimed to establish a method to simulate drug distribution in PLGA drug delivery by using Hoechst 33342 as an imitating drug. Our approach enabled us to identify, isolate, and characterize cells exposed to Hoechst 33342 and to deduce the concentration of this fluorescent dye around both targeted and nontargeted cells. We believe that the method described herein will provide essential information regarding the specificity of cell targeting in any type of PLGA drug delivery system.