Detection of antibodies to Anisakis simplex larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens
Detection of antibodies to Anisakis simplex larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens
复制标题
使用粗制或纯化抗原通过酶联免疫吸附测定和免疫电泳检测单纯异尖线虫幼虫抗体
DOI：
10.1017/s0022149x0000153x
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发表时间：
1994
影响因子：
1.6
通讯作者：
M. Tsuji
中科院分区：
文献类型：
--
作者：
R. Gutierrez Ramos;M. Tsuji
应用酶联免疫吸附试验(EL ISA)和免疫电泳法(IEP)对人和免疫兔的单纯性异尖线虫幼虫感染进行了血清学诊断。粗抗原用Sephacryl S-300型凝胶过滤分离。得到了四个组分。其他蠕虫感染患者的血清有时与异尖线虫幼虫抗原发生交叉反应。对于IEP，粗抗原比纯化抗原更敏感。用酶联免疫吸附试验检测人和免疫兔血清中异尖线虫幼虫抗体时，第三组分最敏感。
Abstract The enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were used for the serodiagnosis of larval Anisakis simplex infections in man and immunized rabbits. Sephacryl S-300 gel filtration was used for separating crude antigen. Four fractions were obtained. Sera from patients with other helminth infections sometimes cross reacted with Anisakis larval antigens. With IEP, crude antigen is more sensitive than purified antigens. With ELISA, the third fraction is the most sensitive for detecting antibodies to Anisakis larvae in the sera of humans and immunized rabbits.
通过海洋食品传播的人类异尖线虫病。
DOI：
--
发表时间：
1991
期刊：
Hawaii medical journal
影响因子：
--
作者：
Deardorff,TL;Kayes,SG;Fukumura,T
通讯作者：
Fukumura,T