Bioluminescence imaging using d-luciferin and its analogs for visualizing Bdnf expression in living mice; different patterns of bioluminescence signals using distinct luciferase substrates
Bioluminescence imaging using d-luciferin and its analogs for visualizing Bdnf expression in living mice; different patterns of bioluminescence signals using distinct luciferase substrates
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DOI：
10.1093/jb/mvac070
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发表时间：
2022-09-01
影响因子：
2.7
通讯作者：
Mori, Hisashi
中科院分区：
文献类型：
--
作者：
Fukuchi, Mamoru;Mitazaki, Satoru;Mori, Hisashi
脑源性神经营养因子（BDNF）在许多脑功能中起着至关重要的作用，包括记忆巩固。以前，我们产生了一个Bdnf-荧光素酶转基因（Bdnf-Luc）小鼠品系，在体内生物发光成像可视化Bdnf表达的变化。我们成功地可视化活性依赖性BDNF诱导活小鼠大脑中使用的d-tetrain类似物，TokeOni，它分布到大脑，并产生近红外生物发光。在这项研究中，我们比较了Bdnf-Luc小鼠全身的生物发光信号模式，这些信号是由d-tetrain，TokeOni和seMpai产生的，seMpai是另一种产生近红外光的d-tetrain类似物。如最近报道的，当使用TokeOni时，在野生型小鼠中观察到肝脏背景信号。当使用d-精氨酸和TokeOni时，从包含肝脏的区域强烈观察到生物发光信号。此外，我们在使用TokeOni时检测到来自大脑的信号。与d-glycin和TokeOni相比，seMpai在Bdnf-Luc小鼠全身广泛检测到信号。seMpai产生的信号在含有骨骼肌的区域特别强。综上所述，当使用不同的荧光素酶底物时，Bdnf-Luc小鼠中的生物发光信号的模式不同。因此，可以通过选择适当的底物来观察BDNF在感兴趣的组织和器官中的表达。
Brain-derived neurotrophic factor (BDNF) plays a crucial role in numerous brain functions, including memory consolidation. Previously, we generated a Bdnf-Luciferase transgenic (Bdnf-Luc) mouse strain to visualize changes in Bdnf expression using in vivo bioluminescence imaging. We successfully visualized activity-dependent Bdnf induction in living mouse brains using a d-luciferin analog, TokeOni, which distributes to the brain and produces near-infrared bioluminescence. In this study, we compared the patterns of bioluminescence signals within the whole body of the Bdnf-Luc mice produced by d-luciferin, TokeOni and seMpai, another d-luciferin analog that produces a near-infrared light. As recently reported, hepatic background signals were observed in wild-type mice when using TokeOni. Bioluminescence signals were strongly observed from the region containing the liver when using d-luciferin and TokeOni. Additionally, we detected signals from the brain when using TokeOni. Compared with d-luciferin and TokeOni, signals were widely detected in the whole body of Bdnf-Luc mice by seMpai. The signals produced by seMpai were strong in the regions containing skeletal muscles in particular. Taken together, the patterns of bioluminescence signals in Bdnf-Luc mice vary when using different luciferase substrates. Therefore, the expression of Bdnf in tissues and organs of interest could be visualized by selecting an appropriate substrate.