Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits.
Extraction, purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits.
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DOI：
10.1016/j.carbpol.2012.11.060
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发表时间：
2013-02
影响因子：
11.2
通讯作者：
Hongjun Wang;Hong Jiang;Shude Wang;X. Li;D. Yao;Jingjing Dong;Tiezhong Zhou;Yingzi Liu;Shenyang Gao;Li Li-Li;Xuming Deng
中科院分区：
文献类型：
--
作者：
Hongjun Wang;Hong Jiang;Shude Wang;X. Li;D. Yao;Jingjing Dong;Tiezhong Zhou;Yingzi Liu;Shenyang Gao;Li Li-Li;Xuming Deng
本研究采用基于单因素实验的响应面法和中心组合设计对南五味子多糖的提取参数（提取时间、提取温度、料液比）进行优化。提取温度和料液比对提取率影响较大。最佳条件为固液比1:27（质量/体积）、提取时间3h、提取温度98℃。在此条件下，粗南五味子多糖(KPS)的最大得率为2.7735±0.068%，与模型预测相符。通过二乙氨基乙基 (DEAE) 纤维素-52 柱色谱法获得五个主要组分：KPS I、KPS II、KPS III、KPS IV 和 KPS V。通过 Sephadex G-100 柱色谱法从 KPS III 中分离出主要组分 KPS III-1。 KPS III-1的气相色谱初步表征表明其主要成分为木糖和半乳糖醛酸。
In this study, response surface methodology and central composite design based on single-factor experiments were applied to the optimization of the extraction parameters (extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from Kadsura marmorata fruits. The extraction temperature and solid–liquid ratio affected the extraction yield significantly. The optimal conditions included a solid–liquid ratio of 1:27 (mass/volume), an extraction time of 3h and an extraction temperature of 98°C. Under these conditions, the maximal yield of crude Kadsura polysaccharide (KPS) was 2.7735±0.068%, which agreed with model predictions. Five major fractions, KPS I, KPS II, KPS III, KPS IV and KPS V, were obtained by diethylaminoethyl (DEAE) cellulose-52 column chromatography. The main fraction, KPS III-1, was isolated from KPS III by Sephadex G-100 column chromatography. Preliminary characterization of KPS III-1 by gas chromatography indicated that it was composed mainly of xylose and galacturonic acid.