INDUCERS OF INTERFERON AND HOST RESISTANCE .2. MULTISTRANDED SYNTHETIC POLYNUCLEOTIDE COMPLEXES
INDUCERS OF INTERFERON AND HOST RESISTANCE .2. MULTISTRANDED SYNTHETIC POLYNUCLEOTIDE COMPLEXES
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DOI：
10.1073/pnas.58.3.1004
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
HILLEMAN, MR
中科院分区：
文献类型：
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作者：
FIELD, AK;TYTELL, AA;HILLEMAN, MR
(b)兔中诱导的动力学：I：C诱导干扰素的动力学如图1所示。干扰素在1小时内出现显著水平，此后不久达到峰值，并在4 - 6小时内显著下降。(2)复合多核苷酸在兔体内诱导的干扰素的表征：注射活性复合多核苷酸的兔血清中的病毒抑制物质基于其生物学和生化性质被鉴定为干扰素。(a)宿主种属特异性：如前所述制备表2所示的兔血清干扰素。小鼠干扰素由每组25只小鼠制备，16-18克重，以0.2毫升剂量静脉注射10.5 μ g的I：C。在两小时内对小鼠放血，合并血清，通过紫外线照射灭菌，并通过针对VSV攻击的空斑减少技术滴定。当血清样品中残留的诱导剂可能干扰种属特异性测定时，用CM-Sephadex层析纯化干扰素。从表2中可以看出，在同源细胞系统中，相应的干扰素引起了抗性，但在异源细胞培养系统中没有。(b)胰蛋白酶敏感性：表3显示兔血清干扰素的活性被胰蛋白酶破坏。(c)分子量：用Sephadex G-200凝胶过滤法测定了I：C诱导的兔血清干扰素的分子量，其范围为49，000 ~ 52，000。（d）等电点：用CM-Sephadex柱层析法测定了I：C诱导的兔血清干扰素的等电点，其pH值为
(b) Kinetics of induction in rabbits: The kinetics for interferon induction by I: C are shown in Figure 1. A significant level of interferon appeared by one hour, reached a peak shortly thereafter, and declinedsignificantly by four to six hours.(2) Characterization of interferon induced in rabbits by complexed polynucleotides: The viral inhibitory substances in the sera of rabbits injected with the active complexed polynucleotides were identified as interferon based on their biological and biochemical properties.(a) Host species-specificity: The rabbit serum interferon noted in Table 2 was prepared as previously described.'Mouse interferon was prepared from groups of 25 mice each, 16-18 gm in weight, injected intravenously with 10.5 jg of I: C in 0.2-ml doses. The mice were bled in two hours, the sera pooled, sterilized by ultraviolet irradiation, and titrated by the plaque reduction technique against VSV challenge. Where the presence of residual inducer in serum samples might interfere with the species-specificity determination, the interferon was purifiedby chromatography on CM-Sephadex. 6 It is seen in Table 2 that resistance was evoked in the homologous cell systems by the corresponding interferons but not in heterologous cell culture systems.(b) Trypsin sensitivity: Table 3 shows that the activity of the rabbit serum interferon was destroyed by trypsin.(c) Molecularweight: Themolecular weight of rabbit serum interferon induced by I: C was determined bygel filtration through Sephadex G-200 and was found to rangefrom 49,000 to 52,000.1 (d) Isoelectric point: The isoelectric point of rabbit serum interferon induced by I: C was measured by chromatography on CM-Sephadex and was found to be pH