Microsatellite (GT)n polymorphism at 3′UTR of SLC11A1 influences the expression of brucella LPS induced MCP1 mRNA in buffalo peripheral blood mononuclear cells
Microsatellite (GT)n polymorphism at 3′UTR of SLC11A1 influences the expression of brucella LPS induced MCP1 mRNA in buffalo peripheral blood mononuclear cells
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DOI：
10.1016/j.vetimm.2012.12.015
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发表时间：
2013-04-15
影响因子：
1.8
通讯作者：
Mitra, Abhijit
中科院分区：
文献类型：
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作者：
Balasubramaniam, Sivamani;Kumar, Subodh;Mitra, Abhijit
溶质载体家族SLC 11 A1基因3 'UTR（GT）n微卫星多态性与牛布鲁氏菌病天然抗性相关。还假设SLC 11 A1对影响宿主抗性的其他候选基因（包括单核细胞趋化/趋化蛋白1（MCP 1））的多效性效应。在本研究中，我们报告了水牛碱（bu）MCP 1的完整编码序列的克隆和特性及其在转录水平上的组织分布。buMCP 1与牛和其他家畜物种同源物的序列同源性高达99%和>80%。buMCP 1 mRNA在不同组织中丰富：在肝脏和乳腺中最丰富，在卵巢、骨骼肌和睾丸中中等，在子宫中最少。此外，定量实时PCR（RTqPCR）分析显示，携带所谓的抗性GII 3等位基因的PBMC内源性产生更多的MCP 1 mRNA，以及当用布鲁氏菌LPS诱导时，表明SLC 11 A1在赋予对细胞内病原体特别是对布鲁氏菌病的抗性中的多效性作用。然而，3 'UTR SLC 11 A1伴随增加趋化因子如MCP 1的产生的潜在分子机制尚未研究。(C)2013爱思唯尔有限公司版权所有。
A (GT)n microsatellite polymorphism at 3'UTR of SLC11A1(solute carrier family 11A1) is associated with the natural resistance to bovine brucellosis. A pleiotropic effect of SLC11A1 on other candidate genes influencing the host resistance including monocyte chemotactic/chemoattractant protein 1 (MCP1) is also hypothesized. In the present study, we report the cloning and characterization of the complete coding sequence of bubaline (bu) MCP1 and its tissue distribution at the transcript level. The buMCP1 exhibited as high as 99% and >80% of sequence identities with the bovine and other domestic animal species homologues. The buMCP1 mRNA was abundant across the different tissues: most abundant in liver and mammary gland, moderate in ovary, skeletal muscle and testis, and least in uterus. Further, quantitative real-time PCR (RTqPCR) analysis revealed that PBMCs carrying so called resistant GII3 allele produced more MCP1 mRNA endogenously as well as when induced with brucella LPS suggesting the pleiotropic roles of SLC11A1 in conferring resistance against the intracellular pathogens particularly against brucellosis. However, the underlying molecular mechanisms by which 3'UTR SLC11A1 concomitantly increases the production of chemokines like MCP1 are yet to be investigated. (C) 2013 Elsevier B.V. All rights reserved.