Neuropeptide Y and calcitonin gene-related peptide modulate voltage-gated Ca2+ channels in mature female rat paracervical ganglion neurons
Neuropeptide Y and calcitonin gene-related peptide modulate voltage-gated Ca2+ channels in mature female rat paracervical ganglion neurons
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DOI：
10.1016/s1071-5576(96)00041-x
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发表时间：
1996-11-01
期刊：
影响因子：
--
通讯作者：
Lewis, DL
中科院分区：
文献类型：
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作者：
Cohen, DP;Ikeda, SR;Lewis, DL
目的：为了解雌性大鼠海马旁神经节（PG）神经元钙通道的主要亚型以及神经活性肽对总钙电流的调节作用，采用全细胞膜片钳技术，观察了选择性钙通道亚型抑制剂和神经活性肽对PG神经元钙电流的调节作用。细胞的数字成像分析用于确定神经节内神经元细胞的大小分布。结果：PG细胞平均直径为28.1 μ m。大多数PG神经元（64%）有N型钙电流，占总钙电流的41%。不存在低电压激活或T型电流。极少数神经元（22%）对P型钙通道阻滞剂欧米茄毒素IVA敏感，只有10%的神经元对L型钙通道阻滞剂尼莫地平敏感。神经肽Y（NPY）对79%的神经元的钙电流有41%的抑制作用，而血管活性肠肽（VIP）则无此作用。降钙素基因相关肽（CGRP）的增加和减少的钙电流在单独的细胞population.CONCLUSIONS：钙电流在雌性大鼠PG神经元进行主要是通过N型钙通道与L-和P-型通道的一个小的贡献。还存在未识别的钙通道类型。说明了NPY对钙电流的调节，并提出了对局部CGRP介导回路存在的支持。版权所有（C）1996年由妇科调查协会。
OBJECTIVE: To assess the predominant subtype of calcium channel present in neurons of the paracervical ganglia (PG) of the female rat and the ability of neuroactive peptides to modulate total calcium current.METHODS: Whole-cell patch clamp techniques were used on isolated PG neurons to assess calcium current modulation in the presence and absence of selective calcium channel subtype inhibitors and neuropeptides. Digital imaging analysis of cells was used to determine neuronal cell size distributions within the ganglia.RESULTS: Average PG cell diameter was 28.1 mu m. Most PG neurons (64%) had an N-type calcium current that contributed 41% for the total calcium current. The low voltage-activated or T-type current was not present. Very few neurons (22%) were sensitive to the P-type calcium channel blocker omega-agatoxin IVA, and in only 10% of neurons was the calcium current sensitive to the L-type channel blocker nimodipine. Neuropeptide Y (NPY) inhibited the calcium current by 41% in 79% of the neurons, but vasoactive intestinal peptide (VIP) has no effect. Calcitonin gene-related peptide (CGRP) both increased and decreased the calcium current in separate cell population.CONCLUSIONS: Calcium currents in female rat PG neurons are carried primarily through N-type calcium channels with a small contribution from L- and P-type channels. An unidentified calcium channel type is also present. Modulation of the calcium current by NPY is demonstrated, and support for the presence fo a local, CGRP-mediated circuit is presented. Copyright (C) 1996 by the Society for Gynecologic Investigation.