Yeast frameshift suppressor mutations in the genes coding for transcription factor Mbf1p and ribosomal protein S3: evidence for autoregulation of S3 synthesis.
Yeast frameshift suppressor mutations in the genes coding for transcription factor Mbf1p and ribosomal protein S3: evidence for autoregulation of S3 synthesis.
复制标题
编码转录因子 Mbf1p 和核糖体蛋白 S3 的基因中的酵母移码抑制突变：S3 合成自动调节的证据。
DOI：
10.1093/genetics/157.3.1141
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发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Culbertson,MR
中科院分区：
文献类型：
--
作者：
Hendrick,JL;Wilson,PG;Edelman,II;Sandbaken,MG;Ursic,D;Culbertson,MR
SUF13和SUF14基因在+1移码突变的基因外抑制子中被鉴定。SUF13与MBF1同义，MBF1是编码POLII转录因子的单拷贝非必需基因。 suf13-1 突变是 SUF13/MBF1 编码区中的两个核苷酸缺失。 Asuf13::TRP1null 突变体抑制 +1 移码突变，表明抑制是由 SUF13 功能丧失引起的。 suf13-1 抑制剂改变对氨基糖苷类抗生素的敏感性并减少 his4-713mRNA 的积累，表明抑制是在翻译水平介导的。 SUF14 基因与 RPS3 同义，RPS3 是编码核糖体蛋白 S3 的单拷贝必需基因。 suf14-1 突变是编码区的错义替换。 S3 表达的增加限制了 SUF14mRNA 的积累，表明表达是自动调节的。 SUF14 中阻止全长翻译的移码突变消除了调节，表明 S3 是调节所必需的。使用 CUP1-SUF14 和 SUF14-lacZ 融合、连续转录测定和 mRNA 半衰期估计，我们的结果表明，转录在调节中起着次要作用（如果有的话），并且 5'-UTR 对于调节来说是必要的，但还不够。 mRNA 衰减率的变化可能是调节的主要机制。
TheSUF13andSUF14genes were identified among extragenic suppressors of +1 frameshift mutations.SUF13is synonymous withMBF1, a single-copy nonessential gene coding for a POLII transcription factor. Thesuf13-1mutation is a two-nucleotide deletion in theSUF13/MBF1coding region. Asuf13::TRP1null mutant suppresses +1 frameshift mutations, indicating that suppression is caused by loss ofSUF13function. Thesuf13-1suppressor alters sensitivity to aminoglycoside antibiotics and reduces the accumulation ofhis4-713mRNA, suggesting that suppression is mediated at the translational level. TheSUF14gene is synonymous withRPS3, a single-copy essential gene that codes for the ribosomal protein S3. Thesuf14-1mutation is a missense substitution in the coding region. Increased expression of S3 limits the accumulation ofSUF14mRNA, suggesting that expression is autoregulated. A frameshift mutation inSUF14that prevents full-length translation eliminated regulation, indicating that S3 is required for regulation. UsingCUP1-SUF14andSUF14-lacZfusions, run-on transcription assays, and estimates of mRNA half-life, our results show that transcription plays a minor role if any in regulation and that the 5′-UTR is necessary but not sufficient for regulation. A change in mRNA decay rate may be the primary mechanism for regulation.