Two types of potassium channels in murine T lymphocytes
Two types of potassium channels in murine T lymphocytes
复制标题
小鼠 T 淋巴细胞中的两种钾通道
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
M. Cahalan
中科院分区：
文献类型：
--
作者：
T. DeCoursey;K. Chandy;S. Gupta;M. Cahalan
本文报道了用吉高欧姆-密封技术记录的小鼠T淋巴细胞全细胞和分离斑片的两种类型K+通道的特性。I型（代表“lpr基因座”或“大”）通道主要在来自突变型MRL/MpJ-lpr/lpr小鼠的T细胞中表征，其中它们大量存在。n型（“正常”）K+通道丰富，因此最容易在来自四种小鼠品系（MRL-+/+、CBA/J、C57 BL/6 J和BALB/c）的伴刀豆球蛋白A激活的T细胞中进行研究。与n型通道相比，l型通道在大约30 mV的正电位下被激活，并且在复极化时更快地关闭。在维持去极化过程中，l型通道的失活比n型通道更慢，也更不完全，但从失活中恢复得更快，因此在重复脉冲过程中几乎没有失活积累。I型通道具有比n型（12 pS）更高的单位电导（21 pS），并且对外部Co++的阻断不太敏感，但是对外部四乙基铵（TEA）的阻断敏感100倍，与n型的8-16 mM相比，I型通道在50-100 μ M TEA下半阻断. TEA通过降低表观单通道电流幅度来阻断两种类型的通道，其剂量-反应关系与阻断宏观电流的剂量-反应关系相似。小鼠n型K+通道类似于人类T细胞中的K+通道。
The properties of two types of K+ channels in murine T lymphocytes are described on the basis of whole-cell and isolated-patch recordings using the gigohm-seal technique. Type l (standing for "lpr gene locus" or "large") channels were characterized mainly in T cells from mutant MRL/MpJ-lpr/lpr mice, in which they are present in large numbers. Type n ("normal") K+ channels are abundant and therefore most readily studied in concanavalin A-activated T cells from four strains of mice, MRL-+/+, CBA/J, C57BL/6J, and BALB/c. Type l channels, compared with type n, are activated at potentials approximately 30 mV more positive, and close much more rapidly upon repolarization. Type l channels inactivate more slowly and less completely than type n during maintained depolarization, but recover from inactivation more rapidly, so that little inactivation accumulates during repetitive pulses. Type l channels have a higher unitary conductance (21 pS) than type n (12 pS) and are less sensitive to block by external Co++, but are 100-fold more sensitive to block by external tetraethylammonium (TEA), with half- block of type l channels at 50-100 microM TEA compared with 8-16 mM for type n. TEA blocks both types of channels by reducing the apparent single channel current amplitude, with a dose-response relation similar to that for blocking macroscopic currents. Murine type n K+ channels resemble K+ channels in human T cells.
影响因子：
64.8
作者：
DECOURSEY, TE;CHANDY, KG;CAHALAN, MD
通讯作者：
CAHALAN, MD
DOI：
10.1073/pnas.83.2.451
发表时间：
1986
影响因子：
11.1
作者：
L. Schlichter;N. Sidell;S. Hagiwara
通讯作者：
L. Schlichter;N. Sidell;S. Hagiwara
电免疫学：离子通道在免疫系统中的生理作用。
DOI：
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发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Chandy,KG;DeCoursey,TE;Cahalan,MD;Gupta,S
通讯作者：
Gupta,S