Analysis of microRNA-size, small RNAs in Streptococcus mutans by deep sequencing
Analysis of microRNA-size, small RNAs in Streptococcus mutans by deep sequencing
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DOI：
10.1111/j.1574-6968.2011.02441.x
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发表时间：
2012-01-01
影响因子：
2.1
通讯作者：
Hong, Su-Hyung
中科院分区：
文献类型：
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作者：
Lee, Heon-Jin;Hong, Su-Hyung
MicroRNAs （miRNAs）是真核细胞中重要的基因表达调节剂。然而，细菌中相同大小的rna之前并没有被专门讨论过。在这里，我们提供了一个mirna大小的rna （msrna）文库，通过深度测序在变形链球菌中注册。生物信息学分析揭示了900多个msRNA物种。通过定量RT-PCR和Northern blotting验证所选msrna的细胞含量。注册的msrna亚群的高丰度和离散大小表明它们的功能意义，尽管在变形链球菌（龋齿的主要病原体之一）中揭示的RNA物种的确切生物学作用还有待阐明。
MicroRNAs (miRNAs) are important modulators of gene expression in eukaryotic cells. However RNAs of the same size in bacteria have not been specifically discussed previously. Here, we provide a library of miRNA-size RNAs (msRNAs), which were registered by deep sequencing in Streptococcus mutans. Bioinformatic analysis of the whole set revealed more than 900 individual msRNA species. The cellular content of selected msRNAs was verified by quantitative RT-PCR and Northern blotting. The high abundance and discrete size of the subset of registered msRNAs suggest their functional significance, although the precise biological role of the RNA species revealed in S. mutans which is one of the principle causative agents of dental caries, has to be elucidated.