Microfluidic technology for making string-like cell tissues
Microfluidic technology for making string-like cell tissues
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DOI：
10.1109/icsj55786.2022.10034737
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
K. Mogi;H. Kimura;N. Takada
中科院分区：
文献类型：
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作者：
K. Mogi;H. Kimura;N. Takada
近年来，人们的注意力集中在用于在微流体装置中产生用于医疗和制药应用的小器官的技术上。特别是在新药开发的研究领域中，强烈希望将器官装置投入实际使用，因为用于药物功效测试的动物可以用装置中的人工器官代替。另一方面，这些设备的开发需要各种技术来将多种类型的细胞塑造成所需的形状，并将它们放置在微通道内的特定位置。在微通道中操纵尺寸从几微米到几十微米的细胞是困难的，并且通常需要专门的技能来按类型排列细胞。因此，我们设计了一种在微通道中培养定向细胞的简单方法。任何人都可以通过简单地将20 - 100 μL的细胞溶液滴到通道的入口端口上，轻松地在微通道的特定位置排列和培养细胞。我们已经通过在装置中培养HUVEC 3天至1周证明了这种微通道的功能。通过以数μL /min的流速更换培养基，在微通道中温和地培养细胞。3天后，确认排列的细胞通过相邻细胞彼此连接。因此，我们成功地培养细长的细胞组织在低剪切应力的环境中使用我们原来的微流体装置。作为未来的发展，我们计划通过结合多种类型的线状细胞组织来创建人工器官。
In recent years, attention has been focused on techniques for creating small organs in microfluidic devices for medical and pharmaceutical applications. Especially in the research field of new drug development, it is strongly desired to put an organ device into practical use because animals for the drug efficacy test can be replaced with an artificial organ in the device. On the other hand, the development of those devices requires various techniques to shape multiple types of cells into the desired figures and place them in specific locations within the microchannel. Manipulating cells sized from a few micrometers to tens of micrometers in a microchannel is difficult and often requires specialized skills for arranging cells by type. Therefore, we devised a simple method for culturing aligned cells in a microchannel. Anyone can easily align and culture cells at specific position in the microchannel by simply dropping 20 - 100 μL of cell solution onto the inlet port of the channel. We have demonstrated the functionality of this microchannel by culturing HUVECs in the device for 3 days to 1 week. The cells were gently cultured in the microchannel by changing the medium at a flow rate of several μL / min. After 3 days, it was confirmed that the aligned cells were connected to each other by adjacent cells. As a result, we succeeded in culturing elongated cellular tissues in an environment with low shear stress using our original microfluidic device. As a future development, we are planning to create an artificial organ by combining multiple types of string-like cell tissues.