Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer's disease
Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer's disease
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DOI：
10.1016/s0304-3940(01)01636-6
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发表时间：
2001-04-20
影响因子：
2.5
通讯作者：
Markesbery, WR
中科院分区：
文献类型：
--
作者：
Aksenov, MY;Markesbery, WR
在这份报告中，我们比较了6名阿尔茨海默病(AD)患者和6名年龄匹配的对照受试者海马体和小脑中的总硫醇水平和蛋白结合硫醇水平。用5，5‘-二硫代二(2-硝基苯甲酸)法测定，AD患者的海马区和小脑区的巯基(SH)总量无明显变化。用3-(N-马来酰亚氨基-丙酰基)生物细胞素标记法测定，AD组大鼠海马区蛋白结合的SH基团水平较对照组降低。逆转录-聚合酶链式反应分析谷胱甘肽氧化还原系统关键基因的表达，证实了AD海马区谷胱甘肽还原酶和谷胱甘肽过氧化物酶信息的诱导。谷胱甘肽转移酶MU和A4-4消息水平没有变化。这项研究表明，保护性抗氧化剂基因反应不足以抵消AD大脑脆弱区域蛋白质氧化损伤的增加。(C)2001爱思唯尔爱尔兰科学有限公司。保留所有权利。
In this report, we compared total and protein-bound thiol levels in the hippocampus and cerebellum of six Alzheimer's disease (AD) patients and six age-matched control subjects. Total level of sulfhydryl (SH) groups, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) method, was not significantly altered in the hippocampus and cerebellum of AD patients. The level of protein-bound SH groups, determined by labeling with 3-(N-maleimido-propionyl) biocytin, was decreased in the AD hippocampus compared with controls. Reverse transcription-polymerase chain reaction analysis of the expression of key glutathione redox system genes demonstrated the induction of glutathione reductase and glutathione peroxidase messages in the AD hippocampus. Levels of glutathione transferase mu and A4-4 messages were unchanged. This study suggests that protective antioxidant gene responses are insufficient to counteract the increased oxidative damage of proteins in a vulnerable region of the AD brain. (C) 2001 Elsevier Science ireland Ltd. All rights reserved.