Propagation of a calcium pulse between osteoblastic cells.
Propagation of a calcium pulse between osteoblastic cells.
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DOI：
10.1016/s0006-291x(05)81535-9
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发表时间：
1992-08
影响因子：
3.1
通讯作者：
Shen-Ling Xia;Jack Ferrier
中科院分区：
文献类型：
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作者：
Shen-Ling Xia;Jack Ferrier
使用大鼠颅盖细胞在原代培养单层和骨样结节，并分离大鼠骨肉瘤细胞，我们通过激光扫描共聚焦显微镜和荧光指示剂fluo-3/AM，表明细胞的机械扰动导致测量的细胞内钙浓度的瞬时增加（脉冲），从细胞传播到细胞，甚至细胞之间仅通过薄的过程连接。在无钙浴介质中的机械扰动细胞中不发生钙脉冲，在这种条件下也没有脉冲传播。阻断缝隙连接的氟烷可抑制增殖。传播速度不随细胞到细胞的连续步骤而降低。这些观察结果表明存在自我再生的钙信号传导机制，该机制可能基于钙诱导的钙释放的形式。
Using rat calvaria cells in primary culture monolayers and bone-like nodules, and isolated rat osteosarcoma cells, we show via laser scanning confocal microscopy and fluorescent indicator fluo-3/AM, that mechanical perturbation of a cell results in a transient increase (pulse) of measured intracellular calcium concentration that propagates from cell to cell, even between cells connected only by a thin process. The calcium pulse does not occur in the mechanically perturbed cell in calcium-free bathing medium, nor is there pulse propagation under this condition. Halothane, which blocks gap junctions, inhibits propagation. Propagation velocity does not decrease with successive cell to cell steps. These observations suggest the existence of a self-regenerating calcium signaling mechanism that may be based on a form of calcium-induced calcium release.