Kinetics of allergen-induced airway eosinophilic cytokine production and airway inflammation
Kinetics of allergen-induced airway eosinophilic cytokine production and airway inflammation
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DOI：
10.1164/ajrccm.160.2.9809130
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发表时间：
1999-08-01
影响因子：
24.7
通讯作者：
O'Byrne, PM
中科院分区：
文献类型：
--
作者：
Gauvreau, GM;Watson, RM;O'Byrne, PM
呼吸道嗜酸性粒细胞增多是哮喘加重的标志。白细胞介素5(IL-5)、嗜酸性粒细胞趋化因子(Eoaxin)等细胞因子的协同作用以及调节活化、正常T细胞表达和分泌(RANTES)似乎是嗜酸性粒细胞外溢所必需的，包括黏附、趋化和激活。这项研究的目的是描述过敏原诱导的炎症细胞向呼吸道募集的动力学和细胞因子对嗜酸性粒细胞趋化、激活或分解的选择性。8名对吸入变应原有双重反应的特应性哮喘患者完成了稀释剂对照的交叉研究。受试者表现出显著的过敏原诱导的早期和晚期呼吸道哮喘反应(p<；0.001)，痰中嗜酸性粒细胞和异色细胞的数量增加(p<；0.05)。吸入变应原后7h，嗜酸性粒细胞IL-5、嗜酸性粒细胞趋化因子和RANTES免疫阳性的数量增加(p<；0.05)，与激活的嗜酸性粒细胞数量的峰值一致。过敏原攻击后痰细胞IL-10免疫阳性率显著降低(p=0.04)，且与痰嗜酸粒细胞呈负相关(r=-0.34，p=0.02)。这项研究表明，变应原诱导的痰嗜酸细胞增多与嗜酸性粒细胞活化和趋化的特异性细胞因子的存在有关，并提示嗜酸性细胞因子的协同作用可能对激活的嗜酸性粒细胞在变态反应性炎症部位的聚集和调节起重要作用。
Airway eosinophilia is the hallmark of asthma exacerbation. Coordination of cytokines such as interleukin (IL)-5, eotaxin and regulated on activation, normal T-cell expressed and secreted (RANTES) seem to be necessary for eosinophil extravasation including adhesion, chemotaxis, and activation. The purpose of this study was to characterize both the kinetics of allergen-induced inflammatory cell recruitment to the airways and cytokines selective for eosinophil chemotaxis, activation, or resolution. Eight atopic asthmatic individuals demonstrating a dual response to inhaled allergen completed a diluent-controlled crossover study. The subjects showed significant allergen-induced early and late airway asthmatic responses (p < 0.001), and an increase in the number of sputum eosinophils and metachromatic cells (p < 0.05). The number of eosinophils immunopositive for IL-5, eotaxin, and RANTES increased 7 h after allergen inhalation (p < 0.05), coincident with the peak number of activated eosinophils. Sputum cells immunopositive for IL-10 decreased significantly following allergen challenge (p = 0.04), and correlated negatively with sputum eosinophils (r = -0.34, p = 0.02). This study shows that allergen-induced increases in sputum eosinophils are associated with the presence of cytokines specific for the activation and chemotaxis of eosinophils, and suggests that cooperation of eosinophilic cytokines may be important for the accumulation and regulation of activated eosinophils at the site of allergic inflammation.