Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.
Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting.
复制标题
通过免疫沉淀和蛋白质印迹评估 ESCRT 蛋白 CHMP5 对客户蛋白泛素化的活性。
DOI：
10.1007/978-1-4939-9492-2_16
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Adoro,Stanley
中科院分区：
文献类型：
--
作者：
Son,Francheska;Umphred-Wilson,Katharine;Shim,Jae-Hyuck;Adoro,Stanley
The charged multivesicular body protein-5 (CHMP5) is a member of the endosomal-sorting complex required for transport (ESCRT) that controls membrane-scission events in eukaryotic cells. Recent studies have revealed novel functions of CHMP5 beyond its role in the ESCRT machinery, notably as a critical nonenzymatic regulator of the ubiquitination and subsequent degradation of proteins in immune cells. Here we describe an immunoprecipitation and western blot methodology for assessing CHMP5 activity on client protein ubiquitination in T lymphocytes.
影响因子：
30.5
作者：
Adoro, Stanley;Park, Kwang Hwan;Glimcher, Laurie H.
通讯作者：
Glimcher, Laurie H.