Temporal changes of the adrenal endocrine system in a restraint stressed mouse and possibility of postmortem indicators of prolonged psychological stress
Temporal changes of the adrenal endocrine system in a restraint stressed mouse and possibility of postmortem indicators of prolonged psychological stress
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DOI：
10.1016/j.legalmed.2014.03.005
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发表时间：
2014-07-01
期刊：
影响因子：
1.5
通讯作者：
Ogata, Mamoru
中科院分区：
文献类型：
--
作者：
Hayashi, Takahito;Ikematsu, Kazuya;Ogata, Mamoru
通过下丘脑-垂体-肾上腺（HPA）轴和交感神经-肾上腺髓质（SA）轴研究束缚应激小鼠肾上腺内分泌系统的时相变化。1天至3周的束缚应激引起血清ACTH和糖皮质激素水平的显着增加，伴随着肾上腺重量的增加，表明HPA轴的激活。肾上腺皮质胆固醇含量降低，反映糖皮质激素的过量产生。此外，与胆固醇供应有关的肾上腺基因表达，包括清道夫受体B类I型、HMG-CoA还原酶和胆固醇敏感性脂肪酶，在同一时期增加。在4周的应激后，所有这些变化都恢复到对照水平。相反，肾上腺嗜铬粒蛋白A的基因表达，这是共同分泌的儿茶酚胺通过SA轴，增加1天至2周的压力，并减少与3-4周的压力。我们的研究结果表明，分析肾上腺内分泌系统的基础上结合几个标志物检查这里将是有用的，不仅证明长期的心理压力的经验，但也确定其持续时间。(C)2014爱思唯尔爱尔兰有限公司版权所有。
We investigated temporal changes of adrenal endocrine systems through the hypothalamic-pituitary-adrenal (HPA) and sympathetic-adrenomedullary (SA) axis in restraint stressed mice. Restraint stress for I day to 3 weeks caused a significant increase in serum levels of ACTH and glucocorticoids accompanied with an increase in adrenal weights, indicating activation of the HPA axis. Reflecting the overproduction of glucocorticoids, adrenal cholesterol content decreased. Moreover, adrenal gene expression involved in cholesterol supply, including scavenger receptor-class B type I, HMG-CoA reductase, and hormone-sensitive lipase, was increased over the same period. After 4 weeks stress, all of these changes returned to control levels. In contrast, adrenal gene expression of chromogranin A, which is cosecreted with catecholamine via the SA axis, was increased with 1 day to 2 weeks of stress, and decreased with 3-4 weeks of stress. Our results suggest that analyses of adrenal endocrine systems based on the combination of several markers examined here would be useful for not only proving prolonged psychological stress experience but also determining its duration. (C) 2014 Elsevier Ireland Ltd. All rights reserved.