GENE SYNTHESIS MACHINES - DNA CHEMISTRY AND ITS USES
GENE SYNTHESIS MACHINES - DNA CHEMISTRY AND ITS USES
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DOI：
10.1126/science.3863253
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
CARUTHERS, MH
中科院分区：
文献类型：
--
作者：
CARUTHERS, MH
由于核酸化学的最新进展，脱氧寡核苷酸现在可以快速且高产率地合成。关键的创新包括基于二氧化硅的支持物上的固相合成和稳定的脱氧核苷亚磷酰胺作为双功能体的开发。当纳入手动，半自动或自动仪器，这些新的程序可以用来制备探针，混合探针，脱氧寡核苷酸引发DNA合成，类似物的脱氧寡核苷酸，和DNA片段含有超过100个脱氧核苷酸。
Deoxyoligonucleotides can now be synthesized rapidly and in high yield because of recent advances in nucleic acid chemistry. Key innovations include solid-phase synthesis on silica-based supports and the development of stable deoxynucleoside phosphoramidites as synthons. When incorporated into manual, semiautomatic, or automatic instruments, these new procedures can be used to prepare probes, mixed probes, deoxyoligonucleotides for priming DNA synthesis, analogues of deoxyoligonucleotides, and DNA segments containing more than 100 deoxynucleotides.