Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: A study by NMR spectroscopy
Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: A study by NMR spectroscopy
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葡萄球菌核酸酶 N 端大片段揭示的天然部分折叠构象和折叠过程：核磁共振波谱研究
DOI：
10.1016/s0022-2836(03)00660-0
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发表时间：
2003-07-18
影响因子：
5.6
通讯作者：
Wang, JF
中科院分区：
文献类型：
--
作者：
Feng, YG;Liu, DS;Wang, JF
葡萄球菌核酸酶（SNase）的N-末端大片段，SNase 110（1-110个残基），SNase 121。SNase 135（1-135个残基）和片段突变体G88 W110、G88 W121、V66 W110和V66 W121。合奏共存的天然样部分折叠和未折叠的状态观察到的片段。持续的天然的三级相互作用驱动片段处于部分折叠状态，这揭示了天然的β-桶构象。G88 W和V66 W突变调节片段分子中固有的天然样三级相互作用的程度，因此，片段突变体折叠成天然样β亚结构域构象。2 M TMAO（trimethylamineN-oxide）与固有的三级相互作用协同作用，通过改变去折叠自由能促进片段的折叠反应，当链长为135个残基时，观察到类似天然的β亚结构域构象。由于Lys 116-Pro 117的顺式和反式X-脯氨酰键，1-121和1-135片段的不均匀部分折叠构象使片段的折叠途径非唯一。片段的折叠反应可以表征为分级过程。(C)2003爱思唯尔科技有限公司版权所有。
The N-terminal large fragments of staphylococcal nuclease (SNase), SNase110 (1-110 residues), SNase121. (1-121 residues), and SNase135 (1-135 residues), and the fragment mutants G88W110, G88W121, V66W110 and V66W121 were studied by heteronuclear multidimensional NMR spectroscopy. Ensembles of co-existent native-like partially folded and unfolded states were observed for fragments. The persistent native-like tertiary interaction drives fragments to be in partially folded states, which reveal native-like beta-barrel conformations. G88W and V66W mutations modulate the extent of inherent native-like tertiary interaction in fragment molecules, and in consequence, fragment mutants fold into native-like beta-subdomain conformations. In cooperation with the inherent tertiary interaction, 2 M TMAO (trimethylamine N-oxide) can promote the folding reaction of fragments through the changes of unfolding free energy, and a native-like beta-subdomain conformation is observed when the chain length contains 135 residues. Heterogeneous partially folded conformations of 1-121 and 1-135 fragments due to cis and trans X-prolyl bond of Lys116-Pro117 make a non-unique folding pathway of fragments. The folding reaction of fragments can be characterized as a hierarchical process. (C) 2003 Elsevier Science Ltd. All rights reserved.