Electrostatic coating enhances bioavailability of insecticides and breaks pyrethroid resistance in mosquitoes
Electrostatic coating enhances bioavailability of insecticides and breaks pyrethroid resistance in mosquitoes
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DOI：
10.1073/pnas.1510801112
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发表时间：
2015-09-29
影响因子：
11.1
通讯作者：
Farenhorst, Marit
中科院分区：
文献类型：
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作者：
Andriessen, Rob;Snetselaar, Janneke;Farenhorst, Marit
杀虫剂耐药性对疟疾和其他蚊媒疾病的控制构成了重大且日益严重的威胁。我们提出了一种基于静电涂层处理的网的杀虫剂施用新方法，该静电涂层通过极性结合杀虫颗粒。静电网可以有效地容纳少量杀虫剂，并在昆虫接触后提高生物利用度。来自非洲各地的六种抗拟除虫菊酯按蚊品系在静电网或标准长效溴氰菊酯涂层蚊帐 (PermaNet 2.0) 上暴露于相似浓度的溴氰菊酯。世卫组织标准暴露生物测定表明，静电网导致的死亡率明显高于 PermaNet，从而有效打破了蚊子的抵抗力。当使用低 15 倍剂量的溴氰菊酯且暴露时间减少至仅 5 秒时，静电网也会导致耐药蚊子的高死亡率。由于不同类型的颗粒粘附在静电网上，因此也可以使用非拟除虫菊酯杀虫剂。三种杀虫剂类别对伊蚊和库蚊均有效，这表明静电网可用于针对所有主要传播疾病的蚊子群部署多种活性杀虫剂。有前途的应用包括在墙壁或屋檐窗帘以及捕集/污染装置中使用静电涂层。我们的结论是，使用静电粘附颗粒可以提高世界卫生组织推荐的杀虫剂的功效，甚至可以对抗具有抗药性的蚊子。这种创新技术有可能支持非常规杀虫剂类别或其组合的使用，可能在病媒控制操作中管理杀虫剂抗药性方面向前迈出重要一步。
Insecticide resistance poses a significant and increasing threat to the control of malaria and other mosquito-borne diseases. We present a novel method of insecticide application based on netting treated with an electrostatic coating that binds insecticidal particles through polarity. Electrostatic netting can hold small amounts of insecticides effectively and results in enhanced bioavailability upon contact by the insect. Six pyrethroid-resistant Anopheles mosquito strains from across Africa were exposed to similar concentrations of deltamethrin on electrostatic netting or a standard longlasting deltamethrin-coated bednet (PermaNet 2.0). Standard WHO exposure bioassays showed that electrostatic netting induced significantly higher mortality rates than the PermaNet, thereby effectively breaking mosquito resistance. Electrostatic netting also induced high mortality in resistant mosquito strains when a 15-fold lower dose of deltamethrin was applied and when the exposure time was reduced to only 5 s. Because different types of particles adhere to electrostatic netting, it is also possible to apply nonpyrethroid insecticides. Three insecticide classes were effective against strains of Aedes and Culex mosquitoes, demonstrating that electrostatic netting can be used to deploy a wide range of active insecticides against all major groups of disease-transmitting mosquitoes. Promising applications include the use of electrostatic coating on walls or eave curtains and in trapping/contamination devices. We conclude that application of electrostatically adhered particles boosts the efficacy of WHO-recommended insecticides even against resistant mosquitoes. This innovative technique has potential to support the use of unconventional insecticide classes or combinations thereof, potentially offering a significant step forward in managing insecticide resistance in vector-control operations.