Estimating Maximal In Vitro Skin Permeation Flux from Studies Using Non-sink Receptor Phase Conditions.
Estimating Maximal In Vitro Skin Permeation Flux from Studies Using Non-sink Receptor Phase Conditions.
复制标题
根据使用非下沉受体相条件的研究估算最大体外皮肤渗透通量。
DOI：
10.1007/s11095-016-1955-8
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发表时间：
2016
影响因子：
3.7
通讯作者：
Roberts,MichaelS
中科院分区：
文献类型：
--
作者：
Yousef,Shereen;Liu,Xin;Mostafa,Ahmed;Mohammed,Yousuf;Grice,JeffreyE;Anissimov,YuriG;Sakran,Wedad;Roberts,MichaelS
PurposeThis study explored the impact of non-sink receptor conditions on the in vitroskin permeation test（IVPT）and seek to estimate equivalent sink condition IVPT data.MethodsSimulated diffusion model and experimental IVPT data was generated for ethyl salicylate across human epidermal membranes in Franz diffusion cell using six different receptor phases，结果模拟和实验IVPT -时间曲线均受受体相溶解度和受体采样率的显著影响。类似的汇条件等效估计的最大通量，通过非线性回归和调整的线性回归估计的稳态通量的相对饱和度的受体相随时间的四个受体相，其中水杨酸乙酯是相对可溶的。显着较低的稳态通量，发现其他两个阶段，其中水杨酸乙酯是不太可溶的是归因于水溶液边界层effect.ConclusionsNon-sink受体相IVPT数据可以用来获得等效的接收器相IVPT数据的接收器相溶解度和流体动力学是足够的，以尽量减少水扩散层对IVPT数据的影响。
PurposeThis study explored the impact of non-sink receptor conditions on thein vitroskin permeation test (IVPT) and sought to estimate equivalent sink condition IVPT data.MethodsSimulated diffusion model and experimental IVPT data were generated for ethyl salicylate across human epidermal membranes in Franz diffusion cells using six different receptor phases, with a 10 fold variation in ethyl salicylate solubility.ResultsBoth simulated and experimental IVPT – time profiles were markedly affected by receptor phase solubility and receptor sampling rates. Similar sink condition equivalent estimated maximum fluxes were obtained by nonlinear regression and adjustment of linear regression estimates of steady state flux for relative saturation of the receptor phase over time for the four receptor phases in which the ethyl salicylate was relatively soluble. The markedly lower steady - state fluxes found for the other two phases in which ethyl salicylate was less soluble was attributed to an aqueous solution boundary layer effect.ConclusionsNon-sink receptor phase IVPT data can be used to derive equivalent sink receptor phase IVPT data provided the receptor phase solubility and hydrodynamics are sufficient to minimise the impact of aqueous diffusion layers on IVPT data.