Gene expression changes in mononuclear cells in patients with metabolic syndrome after acute intake of phenol-rich virgin olive oil
Gene expression changes in mononuclear cells in patients with metabolic syndrome after acute intake of phenol-rich virgin olive oil
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DOI：
10.1186/1471-2164-11-253
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发表时间：
2010-04-20
期刊：
影响因子：
4.4
通讯作者：
Perez-Jimenez, Francisco
中科院分区：
文献类型：
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作者：
Camargo, Antonio;Ruano, Juan;Perez-Jimenez, Francisco
背景资料：先前的研究表明，与低酚初榨橄榄油相比，急性摄入高酚初榨橄榄油可降低促炎、促氧化和促血栓形成标志物，但仍不清楚其酚类成分的影响是否在体内转录水平上发挥作用。为了实现这一目标，我们的目的是确定基因的表达变化，可以介导的初榨橄榄油酚化合物在human.Results：餐后基因表达微阵列分析进行外周血单核细胞在餐后期间。两个初榨橄榄油为基础的早餐与高（398 ppm）和低（70 ppm）的酚类化合物的含量给予20名患者患有代谢综合征后，双盲，随机，交叉设计。为了消除可能存在于他们日常饮食习惯中的潜在影响，所有受试者在研究期间都遵循类似的低脂、富含碳水化合物的饮食。微阵列分析确定了98个差异表达的基因（79个低表达和19个高表达），当比较高酚橄榄油和低酚橄榄油的摄入量时。这些基因中的许多似乎与肥胖、血脂异常和2型糖尿病有关。在这些基因中，有几个基因似乎参与了转录因子NF-κ B、激活蛋白-1转录因子复合物AP-1、细胞因子、丝裂原活化蛋白激酶MAPK或花生四烯酸途径介导的炎症过程。这项研究表明，摄入富含酚类化合物的初榨橄榄油早餐能够抑制体内几种促炎基因的表达，从而将外周血单核细胞的活性转换为较无害的炎症特征。这些结果至少为地中海国家观察到的心血管疾病风险降低提供了部分分子基础，其中初榨橄榄油是膳食脂肪的主要来源。不可否认，其他生活方式因素也可能有助于降低该地区心血管疾病的风险。
Background: Previous studies have shown that acute intake of high-phenol virgin olive oil reduces pro-inflammatory, pro-oxidant and pro-thrombotic markers compared with low phenols virgin olive oil, but it still remains unclear whether effects attributed to its phenolic fraction are exerted at transcriptional level in vivo. To achieve this goal, we aimed at identifying expression changes in genes which could be mediated by virgin olive oil phenol compounds in the human.Results: Postprandial gene expression microarray analysis was performed on peripheral blood mononuclear cells during postprandial period. Two virgin olive oil-based breakfasts with high (398 ppm) and low (70 ppm) content of phenolic compounds were administered to 20 patients suffering from metabolic syndrome following a double-blinded, randomized, crossover design. To eliminate the potential effect that might exist in their usual dietary habits, all subjects followed a similar low-fat, carbohydrate rich diet during the study period. Microarray analysis identified 98 differentially expressed genes (79 underexpressed and 19 overexpressed) when comparing the intake of phenol-rich olive oil with low-phenol olive oil. Many of these genes seem linked to obesity, dyslipemia and type 2 diabetes mellitus. Among these, several genes seem involved in inflammatory processes mediated by transcription factor NF-kappa B, activator protein-1 transcription factor complex AP-1, cytokines, mitogen-activated protein kinases MAPKs or arachidonic acid pathways.Conclusion: This study shows that intake of virgin olive oil based breakfast, which is rich in phenol compounds is able to repress in vivo expression of several pro-inflammatory genes, thereby switching activity of peripheral blood mononuclear cells to a less deleterious inflammatory profile. These results provide at least a partial molecular basis for reduced risk of cardiovascular disease observed in Mediterranean countries, where virgin olive oil represents a main source of dietary fat. Admittedly, other lifestyle factors are also likely to contribute to lowered risk of cardiovascular disease in this region.