Development of a fast responsive and highly sensitive fluorescent probe for Cu2+ ion and imaging in living cells
Development of a fast responsive and highly sensitive fluorescent probe for Cu2+ ion and imaging in living cells
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开发快速响应且高灵敏度的 Cu2 离子荧光探针和活细胞成像
DOI：
10.1016/j.dyepig.2014.02.022
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发表时间：
2014-08-01
影响因子：
4.5
通讯作者：
Zeng, Wenbin
中科院分区：
文献类型：
--
作者：
Huang, Jiaguo;Tang, Min;Zeng, Wenbin
本文报道了一种新型的含丹磺酰基和吡啶基团的荧光猝灭探针（HMPM-NS）。在中性水溶液中，该探针对铜离子的检测比常见的共存金属离子或阴离子具有更高的选择性和灵敏度。基于3 x delta（空白）/k，计算出该探针的检测限低至0.168 μ M的Cu 2+。HMPM-NS与Cu ~（2+）的相互作用模式为1：1。它可以在几乎整个碱性pH窗口（pH 4.8-13.5）中使用，与Cu 2+反应时具有快速响应。由于其优异的光谱特性，我们通过三维细胞成像证明了该探针具有良好的细胞渗透性，并成功地将其应用于活细胞中Cu 2+的可视化，进一步揭示了其在生物系统中的应用价值。(C)2014爱思唯尔有限公司版权所有。
A novel fluorescent quenching probe (HMPM-NS) bearing a dansyl and pyridine group has been successfully developed. This probe showed high selectivity and sensitivity for the detection of copper ion over commonly coexistent metal ions or anions in neutral aqueous media. The limit of detection of this probe based on 3 x delta(blank)/k was calculated as low as 0.168 mu M of Cu2+. And the interaction mode with 1:1 stoichiometry was proposed between HMPM-NS and Cu2+. It can be used in almost the entire alkalinity pH window (from pH 4.8-13.5) with a fast response when reacts with Cu2+. On the account of its excellent spectroscopic properties, We demonstrated this probe displays good cell permeability by 3D cell imaging and then successfully applied it to visualize Cu2+ in living cells, which further revealed its valuable applications in biological systems. (C) 2014 Elsevier Ltd. All rights reserved.