Phospholipid-Graphene Nanoassembly as a Fluorescence Biosensor for Sensitive Detection of Phospholipase D Activity
Phospholipid-Graphene Nanoassembly as a Fluorescence Biosensor for Sensitive Detection of Phospholipase D Activity
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DOI：
10.1021/ac300539s
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发表时间：
2012-07-17
影响因子：
7.4
通讯作者：
Jiang, Jian-Hui
中科院分区：
文献类型：
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作者：
Liu, Si-Jia;Wen, Qian;Jiang, Jian-Hui
基于磷脂在非氧化石墨烯表面上的自组装，开发了一种新型磷脂-石墨烯纳米组装体。通过脂质尾部和石墨烯之间的非共价疏水相互作用可以容易地制备纳米组装体，而不破坏石墨烯片内的电子共轭。这赋予了非氧化石墨烯纳米组件所需的电学和光学性质。磷脂涂层为石墨烯提供了优异的生物相容性、易于溶解和受控的表面改性，使纳米组件成为石墨烯生物功能化的有用平台。研究表明，该纳米组件包含双层磷脂和位于疏水内部的还原氧化石墨烯片，从而提供了细胞膜的独特平面模拟。通过在该纳米组件中使用荧光素标记的磷脂，开发了一种用于磷脂酶D活性测定的荧光生物传感器。该生物传感器被证明具有高灵敏度、宽动态范围和0.010 U/L的极低检测限。此外，由于其单步均相测定形式，它表现出出色的稳健性、改进的测定简单性和通量，以及实时监测反应动力学的内在能力。
A novel phospholipid-graphene nanoassembly is developed based on self-assembly of phospholipids on nonoxidative graphene surfaces. The nanoassembly can be prepared easily through noncovalent hydrophobic interactions between the lipid tails and the graphene without destroying the electronic conjugation within the graphene sheet. This imparts the nanoassembly with desired electrical and optical properties With nonoxidative graphene. The phospholipid coating offers excellent biocompatibility, facile solubilization, and controlled surface modification for graphene, making the nanoassembly a useful platform for biofunctionalization of graphene. The nanoassembly is revealed to comprise a bilayer of phospholipids with a reduced graphene oxide sheet hosting in the hydrophobic interior, thus affording a unique planar mimic of the cellular membrane. By using a fluorescein labeled phospholipid in this nanoassembly, a fluorescence biosensor is developed for activity assay of phospholipase D. The developed biosensor is demonstrated to have high sensitivity, wide dynamic range, and very low detection limit of 0.010 U/L. Moreover, because of its single-step homogeneous assay format it displays excellent robustness, improved assay simplicity and throughput, as well as intrinsic ability to real-time monitor the reaction kinetics.