Western blot analysis of the IgG responses of ruminants infected with Neospora caninum and with Toxoplasma gondii
Western blot analysis of the IgG responses of ruminants infected with Neospora caninum and with Toxoplasma gondii
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DOI：
10.1016/s0021-9975(98)80070-4
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发表时间：
1998-07-01
影响因子：
0.8
通讯作者：
Buxton, D
中科院分区：
文献类型：
--
作者：
Harkins, D;Clements, DN;Buxton, D
用免疫印迹法分析了绵羊、山羊和牛皮下接种NC 1株犬新孢子虫速殖子后的IgG抗体反应。检测到针对广泛NC 1速殖子抗原（6.5至80 kDa）的抗体。显性抗体应答针对36.5 - 38、45.5 - 48.5、52 - 53.5、58、58.5、59.5、60.5、62、63.5、64、66.5、67、67.5、68.5和69.5 kDa的蛋白质，血清来自所有三个物种。这些血清也被用来探测刚地弓形虫抗原的印迹，而一些蛋白带被识别，有没有一致性内或动物物种之间。绵羊、山羊和牛经口感染T.用同样的方法分析M3分离株的弓形虫卵囊。检测到一系列S48弓形虫速殖子抗原（11至83 kDa）的抗体。优势抗体反应针对11、16 - 17、21.5、22.5 - 23.5、26 - 28.5、32 - 35、49.5、50.5、53处的蛋白质; 54.5、60。和61 kDa，与来自所有三个物种的血清。这些血清也被用来探测N.犬抗原，检测到对许多抗原的抗体应答，但在动物种属内或动物种属之间几乎没有一致性。(C)1998年W.B.桑德斯有限公司
The IgG antibody responses of sheep, goats and cattle inoculated subcutaneously with live Neospora caninum tachyzoites of the NC 1 isolate were analysed by Western blotting. Antibodies were detected against a wide range of NC 1 tachyzoite antigens (6.5 to 80 kDa). The dominant antibody responses were directed against proteins at 36.5-38, 45.5-48.5, 52-53.5, 58, 58.5, 59.5, 60.5, 62, 63.5, 64, 66.5, 67, 67.5, 68.5 and 69.5 kDa, with sera from all three species. These sera were also used to probe blots of Toxoplasma gondii antigen and, while a number of protein bands were recognized, there was no consistency within or between animal species. The IgG antibody responses of sheep, goats and cattle orally infected with T. gondii oocysts of the M3 isolate were analysed by the same methods. Antibodies were detected to a range of S48 toxoplasma tachyzoite antigens (11 to 83 kDa). The dominant antibody responses were directed against proteins at 11, 16-17, 21.5, 22.5-23.5, 26-28.5, 32-35, 49.5, 50.5, 53; 54.5, 60. and 61 kDa, with sera from all three species. These sera were also used to probe blots of N. caninum antigen, antibody responses to numerous antigens were detected but showed little consistency within or between animal species. (C) 1998 W.B. Saunders Company Limited.