Passive immunization in infectious bursal disease virus infected birds using chemically purified immune yolk immunoglobulins (IgY)
Passive immunization in infectious bursal disease virus infected birds using chemically purified immune yolk immunoglobulins (IgY)
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DOI：
10.5897/ajmr12.049
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发表时间：
2012-03-30
影响因子：
--
通讯作者：
Anwar, Zubair
中科院分区：
文献类型：
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作者：
Farooq, Ammara;Rabbani, Masood;Anwar, Zubair
传染性法氏囊病是家禽的重要病毒性致死病之一。本研究旨在检测鸡传染性法氏囊病（IBD）病毒免疫卵黄的效价，并从免疫鸡蛋黄中纯化卵黄免疫球蛋白。在该大学的家禽棚里饲养了20只商品蛋鸡，每26周龄间隔15天接种两次油基灭活Gumboro疫苗，获得免疫蛋黄。每隔两周收集一次卵，直至加强后两个半月。用化学方法纯化免疫卵黄。用间接ELISA试剂盒法检测鸡卵黄及半纯化卵黄IgY中IBD抗体效价。将50只15日龄商品代雏鸡分为8组，在大学鸡舍内饲养至36日龄。第30天攻毒，攻毒后3d给予免疫卵黄和半纯化IgY进行被动治疗。半纯化免疫卵黄抗体滴度在3000左右的鸡，各组死亡率均为20%。结论：半纯化IgY和效价大于4000的免疫卵黄对鸡传染性法氏囊病病毒感染有较好的预防效果，且免疫后2个半月内卵黄中仍保留有保护性抗体效价。
Infectious bursal disease is one of the most important killer viral disease of poultry. This study was designed for potency testing of immunized yolk in infectious bursal disease (IBD) virus infected birds and to purify yolk immunoglobulin from egg yolk of immunized hens. Twenty commercial layers were raised in poultry shed of the University and were vaccinated with oil based killed Gumboro vaccine twice at an interval of 15 days at the age of 26 weeks interval to get immuned yolk. Eggs were collected at two weeks interval till two and half months after boosting. Immune yolks were purified by chemical means. Antibody titer against IBD in egg yolk and semi purified egg yolk IgY was measured by indirect ELISA kit method. Fifty commercial chicks of 15 days old were divided into eight groups and were reared in poultry shed in the University up to 36 days. The birds were challenged at 30th day and they were provided with passive therapy of immune yolk and semi purified IgY after 3 days of challenge. The birds which received semi purified immune yolk and antibody titre having more or less 3000 showed 20% mortality in each group. In conclusion semi purified IgY and immune yolk having titer more than 4000 were relatively effective in prevention of infectious bursal disease virus infected birds and protective antibody titer remain in yolk for two and half months after killed vaccination.