THE RPSL GENE AND STREPTOMYCIN RESISTANCE IN SINGLE AND MULTIPLE DRUG-RESISTANT STRAINS OF MYCOBACTERIUM-TUBERCULOSIS
THE RPSL GENE AND STREPTOMYCIN RESISTANCE IN SINGLE AND MULTIPLE DRUG-RESISTANT STRAINS OF MYCOBACTERIUM-TUBERCULOSIS
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DOI：
10.1111/j.1365-2958.1993.tb00924.x
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发表时间：
1993-11-01
影响因子：
3.6
通讯作者：
MORRIS, SL
中科院分区：
文献类型：
--
作者：
NAIR, J;ROUSE, DA;MORRIS, SL
最近出现的惰性和快速致命的耐药菌株结核分枝杆菌重新定义结核杆菌耐药的分子机制的兴趣。本文研究了M.通过对链霉素耐药菌株及其链霉素敏感亲本菌株的核糖体S12蛋白编码基因（rpsL基因）的克隆和核苷酸序列分析，我们已经证明了五个单一的Sm（R）M。结核菌株和对多种抗生素敏感性降低的Sm（R）分离株在rpsL基因的密码子43处具有相同的点突变。同一位点的突变在大肠杆菌中赋予Sm（R）。与此相反，另外两个多重耐药M。对Sm具有抗性的结核菌株具有与其药物敏感亲本菌株具有相同核苷酸序列的rpsL基因，表明这些菌株中涉及不同的抗性机制。
The recent emergence of indolent and rapidly fatal drug-resistant strains of Mycobacterium tuberculosis has renewed interest in defining the molecular mechanisms of drug resistance in the tubercle bacilli. In this report, we have examined the mechanism of resistance to streptomycin (Sm) in M. tuberculosis through the cloning and nucleotide sequence analysis of the gene encoding the ribosomal S12 protein (rpsL gene) from streptomycin-resistant strains and their streptomycin-sensitive parental strains. We have demonstrated that five singly Sm(R) M. tuberculosis strains and an Sm(R) isolate that has reduced sensitivity to multiple antibiotics have identical point mutations at codon 43 of the rpsL gene. Mutations at this same site confer Sm(R) in Escherichia coli. In contrast, two other multiple drug-resistant M. tuberculosis strains that are resistant to Sm have rpsL genes that have the same nucleotide sequence as their drug-sensitive parent strains, suggesting that different resistance mechanisms are involved in these strains.