Levels of soluble adhesion molecules are elevated in the cerebrospinal fluid of children with moyamoya syndrome
Levels of soluble adhesion molecules are elevated in the cerebrospinal fluid of children with moyamoya syndrome
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DOI：
10.1097/00006123-200203000-00022
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发表时间：
2002-03-01
期刊：
影响因子：
4.8
通讯作者：
Scott, RM
中科院分区：
文献类型：
--
作者：
Soriano, SG;Cowan, DB;Scott, RM
目的：烟雾综合征的发病机制尚不清楚；然而，先前的研究表明炎症成分。由于黏附分子介导脑缺血时的炎症反应，我们测量了烟雾综合征儿童血清和脑脊液样本中内皮黏附分子、血管细胞黏附分子1型、细胞间黏附分子1型和e -选择素的可溶性同型异构体水平。方法：采集烟雾综合征患儿（n = 20）和先天性脊柱畸形患儿（n = 20）的血清和脑脊液样本。酶联免疫法测定可溶性血管细胞粘附分子1型、细胞间粘附分子1型和e-选择素水平。分析了可溶性黏附分子水平与铃木血管造影分类的相关性。测定脑脊液/血清白蛋白指数，以确定血脑屏障的完整性。结果：与对照组相比，烟雾综合征患儿脑脊液可溶性血管细胞黏附分子1型、细胞间黏附分子1型和e -选择素水平显著升高。烟雾组白蛋白指数为9，显著高于对照组。然而，三种可溶性黏附分子的血清水平无差异，年龄、铃木分类、血清和脑脊液黏附分子水平无相关性。结论：我们的研究显示脑脊液可溶性内皮粘附分子水平升高，表明烟雾综合征患儿存在持续的中枢神经系统炎症，并伴有轻微的血脑屏障损伤。这些可溶性粘附分子可能在临床上作为炎症过程的指标有用，并可能为这种神秘的疾病过程提供一些见解。
OBJECTIVE: The pathogenesis of moyamoya syndrome is unknown; however, previous studies suggested an inflammatory component. Because adhesion molecules mediate inflammation during cerebral ischemia, we measured the levels of soluble isoforms of the endothelial adhesion molecules vascular cell adhesion molecule Type 1, intercellular adhesion molecule Type 1, and E-selectin in serum and cerebrospinal fluid (CSF) samples from children with moyamoya syndrome.METHODS: Serum and CSF samples were obtained from children with moyamoya syndrome (n = 20) and patients with congenital spinal deformities (n = 20). Soluble vascular cell adhesion molecule Type 1, intercellular adhesion molecule Type 1, and E-selectin levels were measured in enzyme-linked immunoassays. The correlation between the levels of soluble adhesion molecules and the Suzuki angiographic classification was analyzed. CSF/serum albumin index values were also measured, to determine the integrity of the blood-brain barrier.RESULTS: Compared with the control group, children with moyamoya syndrome exhibited significantly elevated CSF levels of soluble vascular cell adhesion molecule Type 1, intercellular adhesion molecule Type 1, and E-selectin. The albumin index for the moyamoya group was 9, which was significantly higher than that for the control group. However, there were no differences in the serum levels of the three soluble adhesion molecules and no correlations between age, Suzuki classification, and serum and CSF levels of adhesion molecules.CONCLUSION: Our study demonstrates increased CSF levels of soluble endothelial adhesion molecules, suggesting that children with moyamoya syndrome have ongoing central nervous system inflammation, with slight impairment of the blood-brain barrier. These soluble adhesion molecules may be clinically useful as indicators of this inflammatory process and may provide some insight into this enigmatic disease process.