Prevalence of fowl glioma-inducing virus in chickens of zoological gardens in Japan and nucleotide variation in the env gene
Prevalence of fowl glioma-inducing virus in chickens of zoological gardens in Japan and nucleotide variation in the env gene
复制标题
DOI：
10.1292/jvms.70.469
复制
发表时间：
2008-05-01
影响因子：
1.2
通讯作者：
Umemura, Takashi
中科院分区：
文献类型：
--
作者：
Hatai, Hitoshi;Ochiai, Kenji;Umemura, Takashi
鸡神经胶质瘤诱导病毒（FGV）是禽白血病病毒（ALV）的A亚群，具有神经系统致瘤性。在日本的一个动物园中，大约40%的鸡，包括日本鸡，感染了FGV。由于该动物园是其他动物园的主要日本家禽供应商，因此怀疑FGV感染已在观赏鸡中传播。本研究采用巢式PCR、逆转录巢式PCR和酶联免疫吸附试验对3个动物园的129只鸡进行了检测。巢式PCR检测结果显示，在每个被检测的菜园中，26%至56%的家禽呈阳性。系统发育分析表明，14株ALV的3'非编码区（包括FGV特异性序列）与FGV有较高的同源性，亲缘关系较近。此外，分离株的env基因经常出现核苷酸突变和缺失。这些结果表明，FGV在日本动物园饲养的观赏鸡中流行。
Fowl glioma-inducing virus (FGV), which belongs to subgroup A of avian leukosis virus (ALV), is tumorigenic in the nervous system. In a zoological garden in Japan, approximately 40% of chickens, including Japanese fowls, were infected with FGV. Because this zoological garden plays a role as a major supplier of Japanese fowl for other zoological gardens, FGV infection is suspected to have spread among ornamental chickens. In this study, the prevalence of the disease was examined in a total of 129 chickens in three other zoological gardens by nested polymerase chain reaction (PCR), reverse transcription nested PCR and enzyme-linked immunosorbent assay. Twenty-six to 56 percent of the fowls in each of the examined gardens were positive by nested PCR. The phylogenetic analysis revealed that the 3' untranslated region, including the specific sequence of FGV, of the 14 isolated ALVs showed high sequence identity and a close relationship with FGV. In addition, the env gene of the isolates frequently showed mutations and deletions of nucleotides. These results suggest that FGV is prevalent among ornamental chickens kept in zoological gardens in Japan.