Genome wide responses of murine lungs to dietary alpha-tocopherol.
Genome wide responses of murine lungs to dietary alpha-tocopherol.
复制标题
小鼠肺部对膳食α-生育酚的全基因组反应。
DOI：
10.1080/10715760600935567
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发表时间：
2007
影响因子：
3.3
通讯作者：
Gohil,Kishorchandra
中科院分区：
文献类型：
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作者：
Oommen,Saji;Vasu,VihasT;Leonard,ScottW;Traber,MaretG;Cross,CarrollE;Gohil,Kishorchandra
α-生育酚（α-T）可能通过调节mRNA浓度影响生物学过程。这项研究筛选了15,000个肺mRNA对小鼠饮食α-T的反应。选择肺作为靶器官是因为它受到氧化和炎症应激的周期性变化，α-T参与了它们的调节。该分析鉴定了对由膳食α-T确定的肺α-T状态敏感的10400种mRNA。女性肺转录组似乎比男性肺对α-T状态更敏感。在这里，我们专注于13个细胞骨架基因的诱导饮食α-T，因为它们在男性和女性的肺类似地诱导。通过定量实时聚合酶链反应（qRT-PCR）确认其诱导作用。三种编码蛋白的免疫组织化学分析表明，它们在肺血管和肺泡区域表达。提示肺α-T状态可调节肺的细胞结构。
α-tocopherol (α-T) may affect biological processes by modulating mRNA concentrations. This study screened the responses of ∼15,000 lung mRNAs to dietary α-T in mice. The lung was chosen as the target organ because it is subjected to cyclical variations in oxidant and inflammatory stressors and α-T has been implicated in their modulations. The analysis identified ∼400 mRNAs sensitive to α-T status of lungs determined by dietary α-T. The female lung transcriptome appears to be more sensitive to the α-T status than that of the male lungs. Here, we focus on the induction of 13 cytoskeleton genes by dietary α-T because they were similarly induced in the male and the female lungs. Their inductions were confirmed by quantitative-real-time-polymerase chain reaction (qRT-PCR). Immunohistochemical analyses of three of the encoded proteins suggest that they are expressed in lung vasculature and alveolar regions. The data suggest that the lung α-T status may modulate cytoarchitecture of lungs.