Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis
Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis
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DOI：
10.1021/ja9102698
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发表时间：
2010-02-10
影响因子：
15
通讯作者：
El-Sayed, Mostafa A.
中科院分区：
文献类型：
--
作者：
Kang, Bin;Mackey, Megan A.;El-Sayed, Mostafa A.
通过适当地将金纳米颗粒与特定肽结合，我们成功地将它们选择性地运送到癌细胞的细胞核中。DNA双链断裂的共聚焦显微镜图像显示，金纳米颗粒在癌细胞细胞核的定位破坏了DNA。金纳米粒子对活细胞的实时暗场成像显示，金纳米粒子的核靶向特异性地诱导癌细胞的细胞分裂停止，在有丝分裂发生后，双核细胞形成。流式细胞术结果表明，细胞分裂失败导致癌细胞程序性死亡（凋亡）。这些结果表明，定位于癌细胞细胞核的金纳米颗粒对于理解纳米材料与生命系统之间的相互作用具有重要意义。
By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA. Gold nanoparticle dark-field imaging of live cells in real time revealed that the nuclear targeting of gold nanoparticles specifically induces cytokinesis arrest in cancer cells, where binucleate cell formation occurs after mitosis takes place. Flow cytometry results indicated that the failure to complete cell division led to programmed cell death (apoptosis) in cancer cells. These results show that gold nanoparticles localized at the nuclei of cancer cells have important Implications in understanding the interaction between nanomaterials and living systems.