Genotyping of Toxoplasma gondii isolates from cats in different geographic regions of China
Genotyping of Toxoplasma gondii isolates from cats in different geographic regions of China
复制标题
DOI：
10.1016/j.vetpar.2011.06.013
复制
发表时间：
2011-12-29
影响因子：
2.6
通讯作者：
Lun, Z. R.
中科院分区：
文献类型：
--
作者：
Chen, Z. W.;Gao, J. M.;Lun, Z. R.
采用限制性片段长度多态性（RFLP）技术，对从我国4个不同地区（安徽、湖北、山西和广东）分离的14个猫弓形虫分离株在SAG 2、SAG 3、BTUB、GRA 6、L358、PK 1、c22-8、c29-2和Apico 8个核基因座上的遗传多样性进行了分析。从这14个分离物中鉴定出两种基因型，但它们都不属于典型的遗传类型（I型、II型和III型）。出乎意料的是，在这14个分离株中观察到如此高的相似性，尽管它们的原始区域显著遥远。我们的研究结果有力地表明，该寄生虫的I型，II型和III型的三个传统的克隆谱系可能不是在中国的优势。此外，我们的研究结果表明，T.中国弓形虫可能是高度克隆性的，基因型不典型，毒力较高。(C)2011 Elsevier B. V.保留所有权利。
Fourteen isolates of Toxoplamsa gondii were isolated from cats from 4 different geographic provinces (Anhui, Hubei, Shanxi and Guangdong) in China and their genetic diversity with 8 nuclear loci SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico, was analysed by restriction fragment length polymorphisms (RFLPs). Two genotypes from these 14 isolates were identified but none of them belongs to the typical genetic types (types I, II and III). It is unexpected that such high similarity was observed in these 14 isolates although their original regions are significantly distant. Our results strongly indicate that the three traditional clonal lineages of types I, II and III of this parasite may not be preponderant in China. In addition, our results show that the genotypes of T. gondii in China may be highly clonal with atypical genotypes and higher virulence. (C) 2011 Elsevier B.V. All rights reserved.