Genetic evidence for CheB- and CheR-dependent chemotaxis system in A. tumefaciens toward acetosyringone
Genetic evidence for CheB- and CheR-dependent chemotaxis system in A. tumefaciens toward acetosyringone
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DOI：
10.1016/j.micres.2008.11.001
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发表时间：
2009-01-01
影响因子：
6.7
通讯作者：
Harighi, Behrouz
中科院分区：
文献类型：
--
作者：
Harighi, Behrouz
根癌农杆菌具有变形杆菌a亚群趋化基因的同源基因，包括orf1、orf2、cheY1、chea、cher、cheb、cheY2、orf9和orf10，并被组织在一个操纵子[Wright et at.来自根癌农杆菌的趋化性簇。基因1998；220：83-9]。利用自杀载体pK18mobsaB，通过框内缺失Cheb和Cher基因，获得了两株根癌农杆菌突变体。在蜂群平板和盲井趋化试验中研究了突变体对乙酰丁香酮的化学敏感行为。在所测试的条件下，Cher和Cheb都是正常趋化所必需的。(C)2008年爱思唯尔股份有限公司。版权所有。
Agrobacterium tumefaciens has homologues of chemotaxis genes of the a-subgroup of proteobacteria, which include orf1, orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, and orf10 and organized in one operon [Wright et at. A chemotaxis cluster from Agrobacterium tumefaciens. Gene 1998;220:83-9]. Two mutants of A. tumefaciens were created by in-frame deletions of cheB and cheR using suicide vector, pK18mobsacB. The chemosensory behaviour of mutants studied on swarm plates and in blind-well chemotaxis assay toward acetosyringone. Under the conditions tested, both CheR and CheB were essential for normal chemotaxis. (C) 2008 Elsevier GmbH. All rights reserved.