Giant Drosophila neurons differentiated from cytokinesis-arrested embryonic neuroblasts.
Giant Drosophila neurons differentiated from cytokinesis-arrested embryonic neuroblasts.
复制标题
巨型果蝇神经元从胞质分裂停滞的胚胎神经母细胞分化而来。
DOI：
10.1002/neu.480210310
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Hotta,Y
中科院分区：
文献类型：
--
作者：
Wu,CF;Sakai,K;Saito,M;Hotta,Y
决定神经元分化的内在因素和外在因素的相对作用尚不完全清楚。我们发现，从果蝇原肠分离的神经母细胞即使在细胞分裂受到抑制的情况下，也能够在培养中分化出神经元特异性的特性。由此产生的巨大的多核神经元显示出增厚的轴突，具有各种不同的分支模式。神经元抗原的表达与正常培养神经元相似，接种后两天内注入电流即可诱发出动作电位。这些结果表明，启动基本神经元形态和功能的特定分化程序的因素已经存在于神经母细胞中。该培养系统中体积增大的细胞更容易进行生理和细胞生物学分析，并可能有助于未来对果蝇系统的研究。
The relative contributions of the intrinsic and extrinsic factors in determining neuronal differentiation are not fully understood yet. We found that isolated neuroblasts fromDrosophilagastrulae were able to differentiate neuron‐specific properties in culture even when cell divisions were inhibited. The resultant giant multinucleated neurons displayed thickened neurites with a variety of distinct branching patterns. Neuronal antigens were expressed as in normal cultured neurons, and action potentials could be evoked by current injection within two days after plating. These results indicate that the factors for initiating specific differentiation programs for basic neuronal form and function are present in a neuroblast already. The cells of increased sizes in this culture system are more accessible to physiological and cell biological analyses and could facilitate future studies of theDrosophilanervous system.