Association between changes in molecular biomarkers of cartilage matrix turnover and changes in knee articular cartilage: a longitudinal pilot study
Association between changes in molecular biomarkers of cartilage matrix turnover and changes in knee articular cartilage: a longitudinal pilot study
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DOI：
10.1186/s40634-019-0179-3
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发表时间：
2019-05-03
影响因子：
1.8
通讯作者：
Duda, Georg N.
中科院分区：
文献类型：
--
作者：
Boeth, Heide;Raffalt, Peter C.;Duda, Georg N.
背景骨关节炎的早期发现是迫切需要的，但直到今天仍是不可能的。这项研究的目的是评估软骨更替的分子生物标记物是否与膝关节软骨厚度在两年期间的纵向变化有关，这些人患膝骨性关节炎的风险增加。第二个目的是评估既往膝关节损伤或患者报告的基线结果(BL)是否与关节软骨改变相关。招募了19名有30年常规高冲击训练历史的排球运动员(平均年龄46.5+/-4.9岁，47%为男性)。分别检测血清II型前胶原C肽(CpII)、软骨低聚基质蛋白(COMP)、胶原酶生成的II型胶原羧基末端新表位(SC2C)、软骨中间层蛋白2(CILP-2)、尿中II型胶原C端肽(CTX-II)和II型胶原的胶原酶生成肽(S)。通过磁共振成像评价胫骨软骨变薄、增厚及绝对厚度的变化。通过国际膝关节文件委员会的主观膝关节形态和简明36身体成分评分对BL的主观临床状态进行评估。结果CILP-2在FU时显著升高，并与实验期间软骨绝对厚度的变化呈线性相关。既往损伤是软骨绝对厚度变化增加的预测因子。结论检测软骨生物标记物CILP-2的变化可能是检测膝关节骨关节炎早期进展的一种有效而敏感的方法，因为CILP-2可能与某些患膝骨性关节炎风险增加的个体的软骨厚度减少有关。先前的膝关节损伤可能预示着关节软骨厚度变化的增加。
Background An early detection of Osteoarthritis is urgently needed and still not possible until today. The aim of the study was to assess whether molecular biomarkers of cartilage turnover are associated with longitudinal change in knee cartilage thickness during a 2 year period in individuals with increased risk of developing knee osteoarthritis. A secondary aim was to assess whether prior knee injury or subjective patient-reported outcomes at baseline (BL) were associated with articular cartilage changes. Nineteen volleyball players (mean age 46.5 +/- 4.9 years, 47% male) with a 30-year history of regular high impact training were recruited. The serum biomarkers Cpropeptide of type II procollagen (CPII), cartilage oligomeric matrix protein (COMP), collagenase generated carboxy-terminal neoepitope of type II collagen (sC2C), cartilage intermediate layer protein 2 (CILP-2), and the urine biomarkers C-telopeptide of type II collagen (CTX-II) and collagenase-generated peptide(s) of type II collagen (C2C-HUSA) were assessed at BL and at 2 year follow up (FU). Femorotibial cartilage thinning, thickening and absolute thickness change between BL and FU was evaluated from magnetic resonance imaging. Subjective clinical status at BL was evaluated by the International Knee Documentation Committee Subjective Knee Form and the Short-Form 36 Physical Component Score. Results CILP-2 was significantly higher at FU and linearly associated with the absolute cartilage thickness change during the experimental period. Prior injury was a predictor of increased absolute cartilage thickness change. Conclusion Measuring the change in the cartilage biomarker CILP-2 might be a valid and sensitive method to detect early development of knee osteoarthritis as CILP-2 appears to be related to cartilage thickness loss in certain individuals with increased risk of developing knee osteoarthritis. Prior knee injury may be predictive of increased articular cartilage thickness change.