Bovine Anterior Pituitary Progenitor Cell Line Expresses Interleukin (IL)-18 and IL-18 Receptor
Bovine Anterior Pituitary Progenitor Cell Line Expresses Interleukin (IL)-18 and IL-18 Receptor
复制标题
DOI：
10.1111/j.1365-2826.2008.01779.x
复制
发表时间：
2008-11-01
影响因子：
3.2
通讯作者：
Yamaguchi, T.
中科院分区：
文献类型：
--
作者：
Nagai, Y.;Ogasawara, H.;Yamaguchi, T.
在脑下垂体前叶，炎症介质通过免疫内分泌途径调节细胞功能。最近的研究表明，未分化的干细胞可以作为免疫调节剂。这些研究促使我们建立了牛垂体前叶的祖细胞系，并详细说明了它的功能。首先，我们通过免疫组织化学将白介素18定位于Rathke囊的边缘细胞层，该细胞层被认为包含了出生后脑下垂体的干/祖细胞室。用有限稀释法从单细胞克隆中建立了牛垂体前叶来源的克隆细胞系，命名为牛垂体前叶来源细胞系(BAPC-1)。BAPC-1细胞持续表达IL-18和IL-18受体的mRNAs，并在含有表皮生长因子和碱性成纤维细胞生长因子的培养液中稳定快速生长。该细胞株还表达干/祖细胞相关因子Nanog、Oct-4、ptch1、Nestin、Notch1、Hes1、LRP和Fzd4的mRNAs，以及胚胎垂体相关因子Lhx3、PitX1和Pit-1的mRNAs。BAPC-1细胞核Pit-1、Hes1和β-连环蛋白抗体免疫组化染色阳性。此外，BAPC-1细胞表达IL-1α、IL-6、IL-7、IL-12和IL-15等细胞因子的mRNAs。IL-18刺激BAPC-1细胞后，IL-1α、IL-6、IL-1β和IL-8的mRNAs表达增加。培养第6天，BAPC-1细胞也表达生长激素mRNA。这些结果有力地表明BAPC-1是一种干/祖细胞系，并通过其细胞因子的产生来调节垂体前叶细胞的免疫内分泌功能。
In the anterior pituitary gland, inflammatory mediators regulate cell function through an immuno-endocrine pathway. Recent studies have shown that undifferentiated stem cells act as immunomodulators. These studies prompted us to establish a progenitor cell line from the bovine anterior pituitary gland and to detail its function. First, we localised interleukin (IL)-18 by immunohistochemistry to the marginal cell layer of Rathke's pouch that is assumed to embody a stem/progenitor cell compartment of the postnatal pituitary gland. A cloned anterior pituitary-derived cell line from the bovine anterior pituitary gland was established from single cell clone by the limiting dilution method and was designated as bovine anterior pituitary-derived cell line (BAPC)-1. BAPC-1 cells constantly expressed mRNAs for IL-18 and IL-18 receptor, and grew steadily and rapidly in the medium containing epidermal growth factor and basic fibroblast growth factor. The cell line also expressed the mRNAs for the stem/progenitor cell- related factors such as Nanog, Oct-4, Ptch1, Nestin, Notch1, Hes1, Lrp and Fzd4, and the mRNAs for embryonic pituitary-related factors, such as Lhx3, PitX1 and Pit-1. The nuclei of BAPC-1 were immunostained positively for Pit-1, Hes1 and beta-catenin antibodies. Furthermore, BAPC-1 cells expressed mRNAs for cytokine such as IL-1 alpha, IL-6, IL-7, IL-12 and IL-15. Stimulation of BAPC-1 cells with IL-18 increased expression of mRNAs for IL-1 alpha, IL-6, IL-1 beta and IL-8. At day 6 in culture, BAPC-1 cells also express growth hormone mRNA. These results strongly suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its cytokine production.