Carbonic anhydrase integrated into a multimarker approach for the detection of the stress status induced by pollution exposure in Mytilus galloprovincialis: A field case study
Carbonic anhydrase integrated into a multimarker approach for the detection of the stress status induced by pollution exposure in Mytilus galloprovincialis: A field case study
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DOI：
10.1016/j.scitotenv.2019.06.446
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发表时间：
2019-11-10
影响因子：
9.8
通讯作者：
Lionetto, M. G.
中科院分区：
文献类型：
--
作者：
Caricato, R.;Giordano, M. E.;Lionetto, M. G.
从生态毒理学生物监测和评价应用的角度出发，采用多标记方法研究了生物指示生物紫贻贝肝胰腺中碳酸酐酶(CA)对化学污染的敏感性。这项研究是通过野外移植实验进行的，使用了在两个感兴趣的地区暴露在野外的初始种群的笼子生物：Augusta-Melilli-Priolo，一个重污染的工业场地(意大利西西里东部)和Brucoli(意大利西西里东部)，一个不受任何污染的地区，被选为参考地点。奥古斯塔贻贝的消化腺CA活性和基因表达显著高于关在布鲁氏菌控制区的贻贝。污染部位动物的CA反应与溶酶体的增殖/大小增加、金属硫蛋白的诱导、HIF-α(低氧诱导因子)的上调、与蛋白质代谢相关的代谢变化以及条件因子的变化相平行。生物响应数据与沉积物化学分析和动物软组织中金属残留物浓度的信息相结合。综上所述，研究结果突出了污染暴露后肝胰腺中CaS的诱导，并证明其适合于集成到多标记方法中来检测和表征这种生物指示生物因污染暴露而导致的应激状态。(C)2019爱思唯尔B.V.保留所有权利。
The work was addressed to study the sensitivity of the enzyme carbonic anhydrase (CA) to chemical pollution in the hepatopancreas of the bioindicator organism Mytilus galloprovincialis in the context of a multimarker approach in view of ecotoxicological biomonitoring and assessment application. The study was carried out by means of a transplanting experiment in the field, using caged organisms from an initial population exposed in the field in two areas of interest: Augusta-Melilli-Priolo, an heavy polluted industrial site (eastern Sicily, Italy), and Brucoli (eastern Sicily, Italy) an area not affected by any contamination and selected as a reference site. Mussels in Augusta presented a significant increase in the digestive gland CA activity and gene expression compared to the animals caged in the control site of Brucoli. The CA response in animals from the polluted site was paralleled by proliferation/increase in the size of lysosomes, as assessed by Lysosensor green charged cells, induction of metallothionein, up-regulation of hif-alpha (hypoxia-inducible factor), metabolic changes associated with protein metabolism, and changes in the condition factor. Biological responses data were integrated with information about sediment chemical analysis and metal residue concentration in animal soft tissues.In conclusion, obtained results highlighted the induction of CAs in the hepatopancreas of Mytilus galloprovincialis following to pollution exposure, and demonstrated its suitability to be integrated into a multimarker approach for the detection and characterization of the stress status induced by pollution exposure in this bioindicator organism. (C) 2019 Elsevier B.V. All rights reserved.