Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging
Catechol Patterned Film Enables the Enzymatic Detection of Glucose with Cell Phone Imaging
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DOI：
10.1021/acssuschemeng.1c04896
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发表时间：
2021-10
影响因子：
8.4
通讯作者：
Si Wu;J. Rzasa;Eunkyoung Kim;Zhiling Zhao;Jinyang Li;W. Bentley;N. N. Payne-N.;Xiaowen Shi;G. Payne
中科院分区：
文献类型：
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作者：
Si Wu;J. Rzasa;Eunkyoung Kim;Zhiling Zhao;Jinyang Li;W. Bentley;N. N. Payne-N.;Xiaowen Shi;G. Payne
生物学提供了一系列材料和机制来制造可持续的高性能材料。在这里，我们制备了一种多糖膜，并使用仿生氧化接枝方法将其与氧化还原活性儿茶酚部分图案化。在这项概念验证研究中，我们表明，该薄膜允许通过手机成像报告酶生物传感反应的结果，用于检测食品和饮料产品中的葡萄糖（葡萄糖是高果糖玉米糖浆的标志物; HFCS）。该方法使用葡萄糖脱氢酶（GDH）来赋予分子识别，而NADH产物将其电子转移到图案化到多糖膜上的儿茶酚部分。由于氧化醌态的颜色比还原邻苯二酚态的颜色深，因此可以检测到邻苯二酚模式的电子“积累”。使用市售的手机成像应用程序，我们证明了含HFCs的产品可以与含有蔗糖或人工甜味剂的类似产品区分开来。因此，我们设想这种方法可以允许买家或消费者在现场或家中检测葡萄糖。此外，这项工作进一步说明了基于儿茶酚的材料用于执行基于氧化还原的功能的潜力。
Biology provides an array of materials and mechanisms to create sustainable high-performance materials. Here, we prepared a polysaccharide film and patterned it with redox-active catechol moieties using a biomimetic oxidative grafting method. In this proof-of-concept study, we show that the film allows results from an enzymatic biosensing reaction to be reported through cell phone imaging for the detection of glucose in food and beverage products (glucose is a marker for high-fructose corn syrup; HFCS). This method uses glucose dehydrogenase (GDH) to confer molecular recognition, while the NADH product transfers its electrons to catechol moieties that are patterned onto a polysaccharide film. The “accumulation” of electrons by the catechol pattern can be detected because the oxidized quinone state is darker in color than the reduced catechol state. Using a commercially available cell phone imaging app, we demonstrate that HFCS-containing products can be distinguished from analogous products containing either sucrose or artificial sweeteners. Thus, we envision that this method could allow buyers or consumers to detect glucose on-site or in-home. In addition, this work further illustrates the potential of catechol-based materials for performing redox-based functions.