Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A
Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A
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肌动蛋白细胞骨架负责蛋白磷酸酶抑制剂花萼蛋白 A 诱导的根毛细胞细胞质组织的变化
作者：
E. Yokota;N. Imamichi;M. Tominaga;T. Shimmen
蛋白磷酸酶抑制剂花盏花素A(Calyculin A，CA)在50 nM以上的浓度下抑制了根毛细胞的胞质流动，并诱导了细胞质的显著形态变化：跨空泡束分散，出现球形的细胞质小体。通过药理学分析，探讨了CA引起细胞质形态变化的机制。解聚肌动蛋白和断裂肌动蛋白的药物Latrunculin B和细胞松弛素D分别在高于10 0 nM和5μM的浓度下抑制CA处理细胞中球形小体的形成。相反，微管解聚药物100μM丙氨酰胺对CA形成球形小体无影响。有趣的是，肌球蛋白的抑制剂60 mM 2，3-丁二酮单肟也抑制了CA诱导的细胞质球体的形成。这些结果表明，肌动蛋白细胞骨架与CA诱导的细胞质形态变化密切相关。
SummaryIn root hair cells ofLimnobium stoloniferum, a protein phosphatase inhibitor, calyculin A (CA), at concentrations higher than 50 nM inhibits cytoplasmic streaming and induces remarkable morphological changes in the cytoplasm: the transvacuolar strands disperse and spherical cytoplasmic bodies emerge. The mechanism of the morphological changes of the cytoplasm induced by CA was studied by pharmacological analyses. The formation of spherical bodies in cells treated with CA was suppressed by the actin-depolymerizing and -fragmenting drugs latrunculin B and cytochalasin D at concentrations higher than 100 nM and 5 μM, respectively. In contrast, 100 μM propyzamide, a microtubule-depolymerizing drug, did not affect the formation of spherical bodies by CA. Interestingly, 60 mM 2,3-butanedione monoxime, an inhibitor of myosin, also suppressed the CA-induced formation of cytoplasmic spherical bodies. These results indicate that the actin cytoskeleton is intimately involved in the morphological changes of the cytoplasm induced by CA.
DOI：
10.1016/0006-291x(89)92189-x
发表时间：
1989-03-31
影响因子：
3.1
作者：
ISHIHARA, H;MARTIN, BL;HARTSHORNE, DJ
通讯作者：
HARTSHORNE, DJ
肌球蛋白抑制剂 2,3-丁二酮单肟对裂殖酵母生理学的影响。
DOI：
10.1016/s0171-9335(98)80087-3
发表时间：
1998
期刊：
European journal of cell biology.
影响因子：
--
作者：
Steinberg,G;McIntosh,JR
通讯作者：
McIntosh,JR
蛋白磷酸酶抑制剂、tautomycin 和 calyculin-A 对人血小板蛋白磷酸化和细胞骨架的影响。
影响因子：
1.5
作者：
Kurisaki,T;Taylor,RG;Hartshorne,DJ
通讯作者：
Hartshorne,DJ