Late memory-related genes in the hippocampus revealed by RNA fingerprinting
Late memory-related genes in the hippocampus revealed by RNA fingerprinting
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DOI：
10.1073/pnas.94.18.9669
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发表时间：
1997-09-02
影响因子：
11.1
通讯作者：
Alkon, DL
中科院分区：
文献类型：
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作者：
Cavallaro, S;Meiri, N;Alkon, DL
虽然长期记忆被认为需要基因表达和增加蛋白质合成的细胞程序，但对联想记忆至关重要的蛋白质的身份在很大程度上是未知的。我们使用RNA指纹识别来识别候选记忆相关基因（MRG），这些基因在水迷宫训练大鼠的海马中上调，这是一个与空间学习密切相关的大脑区域。最初的10个候选基因中的两个与RNA指纹图谱有关，即大鼠2型兰尼碱受体和谷氨酸脱氢酶的同源物（EC 1.4.1.3），通过北方印迹分析、逆转录-PCR和原位杂交进行了进一步研究，并证实为具有不同时间和区域表达的MRG。如图所示，连续的RNA筛选可能有助于揭示MRG的谱，因为它们出现在记忆存储的不同领域。
Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were further investigated by Northern blot analysis, reverse transcription-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.