Effects of Quaternary Ammonium Chain Length on Antibacterial Bonding Agents
Effects of Quaternary Ammonium Chain Length on Antibacterial Bonding Agents
复制标题
季铵链长对抗菌粘合剂的影响
DOI：
10.1177/0022034513502053
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发表时间：
2013-10-01
影响因子：
7.6
通讯作者：
Xu, H. H. K.
中科院分区：
文献类型：
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作者：
Li, F.;Weir, M. D.;Xu, H. H. K.
本研究的目的是合成新的季铵甲基丙烯酸酯（QAMs）与系统不同的烷基链长度（CL），并调查，第一次，CL的抗菌效果，细胞毒性，和牙本质粘接剂的粘接强度的影响。合成CL为3至18的QAM，并将其掺入Scotchbond多功能（SBMP）粘合剂中。固化的树脂用变形链球菌接种。在4小时时研究细菌早期附着。2天后测量生物膜菌落形成单位（CFU）。随着CL从3增加到16，最低抑菌浓度和最低杀菌浓度降低了5个数量级。将QAMs转化为SBMP减少了细菌的早期附着，在CL = 16时具有最少的定殖。CL = 16的生物膜CFU比SBMP对照低4log（p < .05）。各组牙本质粘结强度相似（p > .1）。新的抗菌材料具有与商业对照相似的成纤维细胞/成牙本质细胞活力。总之，增加键合剂中新QAM的链长大大增加了抗菌功效。可以实现变形链球菌生物膜CFU减少4个对数，而不影响粘合强度和细胞毒性。新的含QAM的键合剂有希望用于广泛的抑制生物膜的澄清。
The objectives of this study were to synthesize new quaternary ammonium methacrylates (QAMs) with systematically varied alkyl chain lengths (CL) and to investigate, for the first time, the CL effects on antibacterial efficacy, cytotoxicity, and dentin bond strength of bonding agents. QAMs were synthesized with CL of 3 to 18 and incorporated into Scotchbond Multi-Purpose (SBMP) bonding agent. The cured resins were inoculated with Streptococcus mutans. Bacterial early attachment was investigated at 4 hrs. Biofilm colony-forming units (CFU) were measured after 2 days. With CL increasing from 3 to 16, the minimum inhibitory concentration and minimum bactericidal concentration were decreased by 5 orders of magnitude. Incorporating QAMs into SBMP reduced bacterial early attachment, with the least colonization at CL = 16. Biofilm CFU for CL = 16 was 4 log lower than SBMP control (p < .05). All groups had similar dentin bond strengths (p > .1). The new antibacterial materials had fibroblast/odontoblast viability similar to that of commercial controls. In conclusion, increasing the chain length of new QAMs in bonding agents greatly increased the antibacterial efficacy. A reduction in Streptococcus mutans biofilm CFU by 4 log could be achieved, without compromising bond strength and cytotoxicity. New QAM-containing bonding agents are promising for a wide range of restorations to inhibit biofilms.