Splanchnic and pelvic mechanosensory afferents signal different qualities of colonic stimuli in mice
Splanchnic and pelvic mechanosensory afferents signal different qualities of colonic stimuli in mice
复制标题
DOI：
10.1053/j.gastro.2004.04.008
复制
发表时间：
2004-07-01
期刊：
影响因子：
29.4
通讯作者：
Blackshaw, LA
中科院分区：
文献类型：
--
作者：
Brierley, SM;Jones, RCW;Blackshaw, LA
背景和目标：来自结肠的机械感觉信息通过腰内脏神经（LSN）和骶盆神经（PN）传导到脊髓。由每一个通路编码的机械感觉信息的精确性质仍然是难以捉摸的。在这里，我们的特点和直接比较这两个途径的机械敏感性初级传入的属性。研究方法：使用一种新的体外小鼠结肠制备物，记录了来自LSN和PN的机械敏感性初级传入，并基于它们对具有3种不同机械刺激的感受野刺激的反应进行区分：探测（70 mg-4 g）、环形拉伸（1-5 g）和粘膜抚摸（10-1000 mg）。结果：从脊髓外侧核和脊神经核记录到5种不同类型的传入神经。这三类传入（serelum，肌肉和粘膜）保守的两种途径之间，然而，它们各自的比例，感受野分布，和响应特性差异很大。在一般情况下，这3类传入记录从PN响应较低的刺激强度，显示更大的响应幅度，并适应不完全机械刺激相比，他们的LSN同行。此外，LSN和PN各自包含一种特殊类型的传入（肠系膜和肌肉/粘膜），这是其各自通路所独有的。结论：内脏和盆腔路径包含不同的人群的机械敏感性传入。这些传入神经能够检测一系列机械刺激，并单独调整以检测刺激的类型，幅度和持续时间。这些知识有助于我们理解这两种途径在从结肠传递机械信息中所起的作用。
Background & Aims: Mechanosensory information from the colon is conducted via lumbar splanchnic nerves (LSN) and sacral pelvic nerves (PN) to the spinal cord. The precise nature of mechanosensory information encoded by each pathway has remained elusive. Here, we characterize and directly compare the properties of mechanosensitive primary afferents from these 2 pathways. Methods: Using a novel in vitro mouse colon preparation, mechanosensitive primary afferents were recorded from the LSN and PN and distinguished based on their response to receptive field stimulation with 3 distinct mechanical stimuli: probing (70 mg-4 g), circular stretch (1-5 g), and mucosal stroking (10-1000 mg). Results: Five different classes of afferent were recorded from the LSN and PN. Three of these classes of afferent (serosal, muscular, and mucosal) were conserved between both pathways; however, their respective proportions, receptive field distributions, and response properties differed greatly. In general, these 3 classes of afferent recorded from the PN responded to lower stimulation intensities, displayed greater response magnitudes, and adapted less completely to mechanical stimulation compared with their LSN counterparts. In addition, the LSN and PN each contain a specialized class of afferent (mesenteric and muscular/mucosal), which is unique to their respective pathway. Conclusions: The splanchnic and pelvic pathways contain distinct populations of mechanosensitive afferents. These afferents are capable of detecting an array of mechanical stimuli and are individually tuned to detect the type, magnitude, and duration of the stimulus. This knowledge contributes to our understanding of the role that these 2 pathways play in conveying mechanical information from the colon.