The biochemistry and genetics of DNA and anti-DNA antibodies.
The biochemistry and genetics of DNA and anti-DNA antibodies.
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DNA 和抗 DNA 抗体的生物化学和遗传学。
DOI：
10.1007/bf02205549
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发表时间：
1990
影响因子：
3.4
通讯作者：
Stollar,BD
中科院分区：
文献类型：
--
作者：
Stollar,BD
技术的快速发展使人们对核酸结构的多样性、特异性蛋白质-核酸相互作用的结构基础以及抗核酸抗体可变区的遗传编码有了更多的了解。这些进展为了解系统性红斑狼疮（SLE）特征性抗DNA抗体的性质提供了实验途径。到目前为止，知识的进步带来了更精确和更简单的抗DNA抗体测定，其结果可能会影响临床管理。希望更全面的了解将产生更有效和更有选择性的治疗手段。越来越多的证据表明，在正常个体中产生低水平的主要是与变性DNA反应的IgM抗体（1，2）。在SLE患者中，抗体的量增加，抗体主要是IgG类，并且抗体的显著部分与天然的以及变性的DNA反应（3）。此外，SLE的IgG抗体对DNA的亲和力可能高于正常存在的IgM群体（4，5），但这尚未被广泛记录。根本问题依然存在。这两种抗体群是否有关联？是什么刺激了SLE患者的产量增加和阶级转换？这些抗体是如何与发病机制相适应的？
Rapid growth in technology has yielded increased insight into the variety of nucleic acid structures, the structural basis of specific protein-nucleic acid interactions, and the genetic coding of variable regions of anti-nucleic acid antibodies. These advances provide avenues for experiments toward understanding the nature of anti-DNA antibodies that are characteristic of systemic lupus erythematosus (SLE). So far, the advances in knowledge have brought more precise and simpler anti-DNA antibody assays, the results of which may influence clinical management. It is hoped that more complete understanding will yield effective and more selective means of treatment.Accumulating evidence indicates that production of a low level of mainly IgM antibodies that react with denatured DNA occurs in normal individuals (1, 2). In patients with SLE the amounts of antibody are increased, the antibodies are mainly of the IgG class, and a significant fraction of the antibodies react with native as well as denatured DNA (3). In addition, it is likely that the IgG antibodies of SLE have higher affinities for DNA than those of the normally occurring IgM populations (4, 5), but this has not been documented extensively. Fundamental questions remain. Are these two populations of antibodies at all related? What stimulates the increased production and the class switch in SLE patients? How do the antibodies fit into pathogenesis?