Immunohistochemical characterization of epithelial cells implanted in the flap-stroma interface of the cornea
Immunohistochemical characterization of epithelial cells implanted in the flap-stroma interface of the cornea
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DOI：
10.1007/s10384-004-0159-7
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发表时间：
2005-03-01
影响因子：
2.4
通讯作者：
Murakami, A
中科院分区：
文献类型：
--
作者：
Chen, LZ;Kato, T;Murakami, A
目的：探讨角膜瓣-基质角膜界面上皮细胞植入后细胞外基质胶原的表达及其与角膜混浊的关系。方法：在兔眼上制作角膜瓣，机械刮取角膜瓣周围组织上皮细胞，植入角膜瓣下方。手术后 3、7 和 30 天采集角膜。进行组织学和免疫组织化学检查。测定I、III、IV型胶原和明胶酶A的表达和定位。结果：裂隙灯检查显示上皮细胞植入区域角膜混浊。组织学研究显示皮瓣和基质界面之间存在上皮细胞簇。植入后1周和1个月，在鼻内上皮岛的周边检测到IV型胶原蛋白的强烈免疫反应性，但在植入的上皮细胞外的界面中未检测到。在植入的上皮细胞和周围的角膜细胞中检测到明胶酶A的弱阳性染色。结论：植入的上皮细胞周围IV型胶原的大量沉积表明它可能是激光原位角膜磨镶术后观察到的患者界面混浊的重要组成部分。明胶酶 A 也可能在上皮向内生长后基质重塑的调节中发挥作用。 (c) 日本眼科学会 2005 年。
Purpose: To investigate the expression of extracellular matrix collagens and their relationship to corneal opacities after implantation of epithelial cells in the flap-stroma corneal interface.Methods: A corneal flap was made on rabbit eyes, and epithelial cells, mechanically scraped from tissue surrounding the flap, were implanted beneath the flap. The corneas were harvested I 3. 7. and 30 days following a surgery. Histological and immunohistochemical examinations were performed. The expression and localization of types I, III, and IV collagens and gelatinase A were determined.Results: Slit-lamp examination showed corneal opacity in the area where the epithelial cells were implanted. Histological study revealed clusters of epithelial cells between the flap and stromal interface. One week and 1 month after the implantation, intense immunoreactivity for collagen type IV was detected at the perimeters of the intrastronial epithelial islands, but not in the interface Outside the implanted epithelial cells. Weak positive staining for gelatinase A was detected in the implanted epithelial cells and surrounding keratocytes.Conclusions: The heavy deposition of collagen type IV surrounding the implanted epithelial cells indicated that it might be an essential component of the interface haze observed in patients following laser in situ Keratomileusis. Gelatinase A may also play a role in the regulation of stromal remodeling after epithelial ingrowth. (c) Japanese Ophthalmological Society 2005.